BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6605

Title: E. Coli Ada in a complex with DNA   PubMed: 16209950

Authors: He, Chuan; Hus, Jean-Christophe; Sun, Li Jing; Zhou, Pei; Norman, Derek; Doetsch, Volker; Gross, John; Lane, William; Wagner, Gerhard; Verdine, Gregory

Citation: He, Chuan; Hus, Jean-Christophe; Sun, Li Jing; Zhou, Pei; Norman, Derek; Dotsch, Volker; Wei, Hua; Gross, John; Lane, William; Wagner, Gerhard; Verdine, Gregory. "A Methylation-Dependent Electrostatic Switch Controls DNA Repair and Transcriptional Activation by E. coli Ada"  Mol Cell 20, 117-129 (2005).

Assembly members:
Ada, polymer, 139 residues, Formula weight is not available
Ada, polymer, 18 residues, Formula weight is not available
Ada, polymer, 18 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Ada: MKKATCLTDDQRWQSVLARD PNADGEFVFAVRTTGIFXRP SCRARHALRENVSFYANASE ALAAGFRPCKRCQPDKANPR QHRLDKITHACRLLEQETPV TLEALADQVAMSPFHLHRLF KATTGMTPKAWQQAWRARR
Ada: TCTTGCGCTTTAATTTGC
Ada: GCAAATTAAAGCGCAAGA

Data sets:
Data typeCount
13C chemical shifts438
15N chemical shifts142
1H chemical shifts671

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ada1
2Ada2
3Ada3
4Ada4

Entities:

Entity 1, Ada 139 residues - Formula weight is not available

1   METLYSLYSALATHRCYSLEUTHRASPASP
2   GLNARGTRPGLNSERVALLEUALAARGASP
3   PROASNALAASPGLYGLUPHEVALPHEALA
4   VALARGTHRTHRGLYILEPHESMCARGPRO
5   SERCYSARGALAARGHISALALEUARGGLU
6   ASNVALSERPHETYRALAASNALASERGLU
7   ALALEUALAALAGLYPHEARGPROCYSLYS
8   ARGCYSGLNPROASPLYSALAASNPROARG
9   GLNHISARGLEUASPLYSILETHRHISALA
10   CYSARGLEULEUGLUGLNGLUTHRPROVAL
11   THRLEUGLUALALEUALAASPGLNVALALA
12   METSERPROPHEHISLEUHISARGLEUPHE
13   LYSALATHRTHRGLYMETTHRPROLYSALA
14   TRPGLNGLNALATRPARGALAARGARG

Entity 2, Ada 18 residues - Formula weight is not available

1   DTDCDTDTDGDCDGDCDTDT
2   DTDADADTDTDTDGDC

Entity 3, Ada 18 residues - Formula weight is not available

1   DGDCDADADADTDTDADADA
2   DGDCDGDCDADADGDA

Entity 4, Ada - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: Ada, {U-13C; U-15N; U-70% 2H], 0.5 mM; Ada 0.5 mM; Ada 0.5 mM; sodium phosphate 25 mM; NaCl 50 mM; DTT 5 mM

conditions_1: pH: 6.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCsample_1not availableconditions_1
1H13C_HSQC 10% 13Csample_1not availableconditions_1
3D TROSY-HNCA 2H, 13C, 15Nsample_1not availableconditions_1
3D TROSY-HN(CO)CA 2H, 13C, 15Nsample_1not availableconditions_1
3D TROSY-HN(CA)CB 2H, 13C, 15Nsample_1not availableconditions_1
3D TROSY-HN(COCA)CB 2H, 13C, 15Nsample_1not availableconditions_1
3D TROSY-HN(CA)CO 2H, 13C, 15Nsample_1not availableconditions_1
3D TROSY-HNCO 2H, 13C, 15Nsample_1not availableconditions_1
3D H(CC-CO)NH-TOCSY 70% 2H, 15N, 13Csample_1not availableconditions_1
3D (H)C(C-CO)NH-TOCSY 70% 2H, 15N, 13Csample_1not availableconditions_1
3D HCCH-TOCSY 13C 13Csample_1not availableconditions_1
3D 15N-NOESY-HSQC mix=60ms 15Nsample_1not availableconditions_1
3D 13C-NOESY-HSQC mix=100ms 13Csample_1not availableconditions_1
3D 13C-NOESY-HSQC mix=200ms 2H; 13C Methyl group of V,I,L; 1H of F,Ysample_1not availableconditions_1
2D NOESY mix=100ms D2O unlabeled DNA, 2H, 15N protein with only I delta1, L delta1,2 and V gamma1,2 13Csample_1not availableconditions_1
3D 15N-NOESY-HSQC mix=200ms 2H, 15N protein samplesample_1not availableconditions_1
3D 13C-NOESY-HSQC mix=100ms D2O 13C C5A labeled Thymine Top or Bottom Strandsample_1not availableconditions_1
3D 13C-NOESY-HSQC mix=100ms in H2O 13C Methyl group of Cys38sample_1not availableconditions_1

Software:

DYANA v1.5 -

X-PLOR v3.815 -

NMRPipe v2001 -

NMR spectrometers:

  • Varian INOVA 900 MHz
  • Varian INOVA 750 MHz
  • Varian INOVA 500 MHz
  • Bruker AVANCE 500 MHz

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