BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6597

Title: Human ARNT C-terminal PAS domain   PubMed: 16181639

Authors: Card, P.; Erbel, J.; Gardner, K.

Citation: Card, P.; Erbel, P.; Gardner, K.. "Structural Basis of ARNT PAS-B Dimerization: Use of a Common Beta-sheet Interface for Hetero- and Homodimerization."  J. Mol. Biol. 353, 664-677 (2005).

Assembly members:
hARNT, polymer, 119 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
hARNT: GAMDNVCQPTEFISRHNIEG IFTFVDHRCVATVGYQPQEL LGKNIVEFCHPEDQQLLRDS FQQVVKLKGQVLSVMFRFRS KNQEWLWMRTSSFTFQNPYS DEIEYIICTNTNVKNSSQE

Data sets:
Data typeCount
1H chemical shifts862
13C chemical shifts545
15N chemical shifts133

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Aryl hydrocarbon receptor nuclear translocator1

Entities:

Entity 1, Aryl hydrocarbon receptor nuclear translocator 119 residues - Formula weight is not available

1   GLYALAMETASPASNVALCYSGLNPROTHR
2   GLUPHEILESERARGHISASNILEGLUGLY
3   ILEPHETHRPHEVALASPHISARGCYSVAL
4   ALATHRVALGLYTYRGLNPROGLNGLULEU
5   LEUGLYLYSASNILEVALGLUPHECYSHIS
6   PROGLUASPGLNGLNLEULEUARGASPSER
7   PHEGLNGLNVALVALLYSLEULYSGLYGLN
8   VALLEUSERVALMETPHEARGPHEARGSER
9   LYSASNGLNGLUTRPLEUTRPMETARGTHR
10   SERSERPHETHRPHEGLNASNPROTYRSER
11   ASPGLUILEGLUTYRILEILECYSTHRASN
12   THRASNVALLYSASNSERSERGLNGLU

Samples:

sample_1: hARNT, [U-15N; U-13C], 1 mM; Tris buffer 50 mM; NaCl 17 mM; DTT 5 mM; H2O 90%; D2O 10%

sample_2: hARNT, [U-15N], 0.478 mM; Tris buffer 50 mM; NaCl 17 mM; DTT 5 mM; H2O 93%; D2O 7%

sample_cond_1: pH: 6.5; temperature: 298 K; ionic strength: 17 mM; pressure: 1 atm

sample_cond_2: pH: 6.6; temperature: 310 K; ionic strength: 17 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 15N 13C-seperated NOESYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available
HNHAnot availablenot availablenot available
3D HNCACBnot availablenot availablenot available
3D CBCACONHnot availablenot availablenot available
3D HNCOnot availablenot availablenot available
3D 15N-seperated TOCSYnot availablenot availablenot available
3D HCCH TOCSYnot availablenot availablenot available
HBCBCGCDHDnot availablenot availablenot available
IPAP HSQCnot availablenot availablenot available

Software:

NMRPipe v2.3 - processing

NMRView v5.2.2 - data analysis

ARIA v1.2 - structure solution

CNS v1.1 - structure solution, refinement

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 15928
PDB
DBJ BAA19931 BAB40668 BAD93114 BAE16957 BAF02595
EMBL CAC21446 CAD38953 CAH90519 CCV01219
GB AAA51777 AAQ96598 ABG67008 EAW53510 EAW53511
REF NP_001075675 NP_001125275 NP_001166569 NP_001184254 NP_001268346
SP O02748 P27540 Q9BE97
TPG DAA31641