BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6562

Title: A novel cGUUAg tetraloop structure with a conserved yYNMGg-type backbone conformation from cloverleaf 1 of bovine enterovirus 1 RNA   PubMed: 15814817

Authors: Ihle, Yvonne; Ohlenschlager, Oliver; Duchardt, Elke; Ramachandran, Ramadurai; Gorlach, Matthias

Citation: Ihle, Yvonne; Ohlenschlager, Oliver; Hafner, Sabine; Duchardt, Elke; Zacharias, Martin; Seitz, Simone; Zell, Roland; Ramachandran, Ramadurai; Gorlach, Matthias. "A novel cGUUAg tetraloop structure with a conserved yYNMGg-type backbone conformation from cloverleaf 1 of bovine enterovirus 1 RNA"  Nucleic Acids Res. 33, 2003-2011 (2005).

Assembly members:
D-loop from cloverleaf 1 of bovine enterovirus 1 RNA, polymer, 18 residues, Formula weight is not available

Natural source:   Common Name: bovine enterovirus 1   Taxonomy ID: 12064   Superkingdom: Viruses   Kingdom: not available   Genus/species: Enterovirus bovine enterovirus 1

Experimental source:   Production method: enzymatic semisynthesis

Entity Sequences (FASTA):
D-loop from cloverleaf 1 of bovine enterovirus 1 RNA: GGCGUUCGUUAGAACGUC

Data sets:
Data typeCount
13C chemical shifts197
15N chemical shifts93
31P chemical shifts16
coupling constants17

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1BEVSLDap1

Entities:

Entity 1, BEVSLDap 18 residues - Formula weight is not available

1   GGCGUUCGUU
2   AGAACGUC

Samples:

sample_1: D-loop from cloverleaf 1 of bovine enterovirus 1 RNA, [U-15N], 1.0 mM; H2O 90%; D2O 10%; KH2PO4/K2HPO4 10 mM; KCl 40 mM; EDTA 0.2 mM

sample_2: D-loop from cloverleaf 1 of bovine enterovirus 1 RNA, [U-13C; U-15N], 0.9 mM; H2O 90%; D2O 10%; KH2PO4/K2HPO4 10 mM; KCl 40 mM; EDTA 0.2 mM

sample_3: D-loop from cloverleaf 1 of bovine enterovirus 1 RNA, [U-13C; U-15N], 0.9 mM; D2O 99.99%; KH2PO4/K2HPO4 10 mM; KCl 40 mM; EDTA 0.2 mM

sample_cond_1: ionic strength: 50 mM; pH: 6.2; pressure: 1 atm; temperature: 298 K

sample_cond_2: ionic strength: 50 mM; pH: 6.2; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
(1H,15N)-HSQCnot availablenot availablenot available
(1H,15N)-CPMG-NOESYnot availablenot availablenot available
(1H,1H)-H5(C5C4N)Hnot availablenot availablenot available
(1H,1H)-HCCNH-TOCSYnot availablenot availablenot available
(1H,1H)-HCCH-TOCSY (A)not availablenot availablenot available
(1H,1H)-HCCH-COSYnot availablenot availablenot available
(1H,15N)-2bond-HSQCnot availablenot availablenot available
(1H,13C)-H(N)COnot availablenot availablenot available
(1H,13C)-HSQCnot availablenot availablenot available
3D (1H,1H,13C)-HCCH-COSYnot availablenot availablenot available
3D (1H,1H,13C)-HCCH-TOCSYnot availablenot availablenot available
(1H,15N)-HCNnot availablenot availablenot available
3D (1H,1H,13C)-NOESY-HSQCnot availablenot availablenot available
(1H,31P)-HCP-CCH-TOCSYnot availablenot availablenot available
(1H,1H)-NOESYnot availablenot availablenot available
HNN-COSYnot availablenot availablenot available
(1H,13C)-HCCH-E.COSYnot availablenot availablenot available

Software:

VNMR v6.1, rev. c - collection, processing

XEASY v1.3.9 - data analysis

CYANA v1.0.6 - structure solution

FOUND vimplemented in CYANA-1.0.6 - structure solution

OPAL v2.6 - refinement

NMR spectrometers:

  • Varian INOVA 750 MHz
  • Varian INOVA 600 MHz

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