BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6536

Title: 1H,15N,13C backbone and sidechain assignment for human ADAP hSH3-1 domain in reduced form

Authors: Zimmermann, Juergen; Freund, Christian

Citation: Zimmermann, Juergen; Freund, Christian. "NMR assignment of the reduced and oxidized forms of the human ADAP hSH3-1 domain"  J. Biomol. NMR 32, 94-94 (2005).

Assembly members:
hSH3-1 domain, polymer, 96 residues, 11861.6 Da.

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
hSH3-1 domain: EKKEQKEKEKKEQEIKKKFK LTGPIQVIHLAKACCDVKGG KNELSFKQGEQIEIIRITDN PEGKWLGRTARGSYGYIKTT AVEIDYDSLKLKKDLE

Data sets:
Data typeCount
13C chemical shifts239
15N chemical shifts85
1H chemical shifts479

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1hSH3-1 domain1

Entities:

Entity 1, hSH3-1 domain 96 residues - 11861.6 Da.

1   GLULYSLYSGLUGLNLYSGLULYSGLULYS
2   LYSGLUGLNGLUILELYSLYSLYSPHELYS
3   LEUTHRGLYPROILEGLNVALILEHISLEU
4   ALALYSALACYSCYSASPVALLYSGLYGLY
5   LYSASNGLULEUSERPHELYSGLNGLYGLU
6   GLNILEGLUILEILEARGILETHRASPASN
7   PROGLUGLYLYSTRPLEUGLYARGTHRALA
8   ARGGLYSERTYRGLYTYRILELYSTHRTHR
9   ALAVALGLUILEASPTYRASPSERLEULYS
10   LEULYSLYSASPLEUGLU

Samples:

sample_1: SH3 domain, [U-13C; U-15N], 1.0 ± 0.05 mM; NaCl 20 mM; Potassiumdihydrogenphosphate 50 mM; Dithtiothreitol 10 mM

conditions_1: pH: 7.0; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
15N_NOESYsample_1isotropicconditions_1
13C_NOESYsample_1isotropicconditions_1
HNCOsample_1isotropicconditions_1
2D_NOESYsample_1isotropicconditions_1
15N_HSQCsample_1isotropicconditions_1

Software:

AZARA v2.0 - data processing

ANSIG v3.3 - assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

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