BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6505

Title: Solution Structure of the Conserved Protein from the Gene Locus Mm1357 of Methanosarcina Mazei. Northeast Structural Genomics Target Mar30   PubMed: 17496898

Authors: Rossi, P.; Aramini, J.; Swapna, G.; Huang, Y.; Xiao, R.; Ho, C.; Ma, L.; Acton, T.; Montelione, G.

Citation: Aramini, James; Rossi, Paolo; Anklin, Clemens; Xiao, Rong; Montelione, Gaetano. "Microgram-scale protein structure determination by NMR"  Nature Methods 4, 491-493 (2007).

Assembly members:
MM1357, polymer, 68 residues, Formula weight is not available

Natural source:   Common Name: Methanosarcina mazei   Taxonomy ID: 2209   Superkingdom: Archaea   Kingdom: not available   Genus/species: Methanosarcina mazei

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
MM1357: MFREESRSVPVEEGEVYDVT IQDIARQGDGIARIEGFVIF VPGTKVGDEVRIKVERVLPK FAFASVVE

Data sets:
Data typeCount
1H chemical shifts482
13C chemical shifts304
15N chemical shifts64

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hypothetical protein MM13571

Entities:

Entity 1, hypothetical protein MM1357 68 residues - Formula weight is not available

1   METPHEARGGLUGLUSERARGSERVALPRO
2   VALGLUGLUGLYGLUVALTYRASPVALTHR
3   ILEGLNASPILEALAARGGLNGLYASPGLY
4   ILEALAARGILEGLUGLYPHEVALILEPHE
5   VALPROGLYTHRLYSVALGLYASPGLUVAL
6   ARGILELYSVALGLUARGVALLEUPROLYS
7   PHEALAPHEALASERVALVALGLU

Samples:

sample_1: MM1357, [U-13C; U-15N], 2 mM; NaN3 0.02%; DTT 10 mM; CaCl2 5 mM; NaCl 100 mM; MES 20 mM; D2O 5%; H2O 95%

sample_2: MM1357, [U-13C; U-15N], 0.56 mM; NaN3 0.02%; DTT 10 mM; CaCl2 5 mM; NaCl 100 mM; MES 20 mM; D2O 100%

sample_3: MM1357, [U-5% 13C; U-15N], 0.78 mM; NaN3 0.02%; DTT 10 mM; CaCl2 5 mM; NaCl 100 mM; MES 20 mM; D2O 5%; H2O 95%

sample_cond_1: pH: 6.5; temperature: 293 K; ionic strength: 100 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESY (aliph)not availablenot availablesample_cond_1
3D 15N-separated NOESYnot availablenot availablesample_cond_1
Hi-res CH-HSQCnot availablenot availablesample_cond_1
HcCH-COSYnot availablenot availablesample_cond_1
Hi-res CH-HSQC (stereo V and L Meth.)not availablenot availablesample_cond_1
HNHAnot availablenot availablesample_cond_1
HCCH-TOCSY (aliph)not availablenot availablesample_cond_1
HCCH-TOCSY (arom)not availablenot availablesample_cond_1
HNCOCACBnot availablenot availablesample_cond_1
HNCOnot availablenot availablesample_cond_1
HNCAnot availablenot availablesample_cond_1
HBHANHnot availablenot availablesample_cond_1
HNCOCAnot availablenot availablesample_cond_1
HBHACONHnot availablenot availablesample_cond_1
HNCACOnot availablenot availablesample_cond_1
HNCACBnot availablenot availablesample_cond_1
3D 13C-separated NOESY (arom)not availablenot availablesample_cond_1

Software:

VNMR v6.1C - collection

XWINNMR v3.5 - collection

NMRPipe v2.1 - processing

Sparky v3.91 - data analysis

AutoAssign v1.14 - data analysis

AutoStructure v2.1.0 - refinement

NMR spectrometers:

  • Bruker AVANCE 500 MHz

Related Database Links:

PDB
GB AAM03512 AAM31053 AAZ69994 AAZ69995
REF NP_615032 NP_633381 WP_011020117 WP_011033303 WP_011306043