BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6474

Title: The 15N relaxation data and backbone dynamics results of CLV1 pT868 bound KI-FHA from KAPP   PubMed: 16042389

Authors: Ding, Zhaofeng; Lee, Gui-in; Liang, Xiangyang; Gallazzi, Fabio; Van Doren, Steven

Citation: Ding, Zhaofeng; Lee, Gui-In; Liang, Xiangyang; Gallazzi, Fabio; Arunima, A.; Van Doren, Steven. "PhosphoThr Peptide Binding Globally Rigidifies Much of the FHA Domain from Arabidopsis Receptor Kinase-Associated Protein Phosphatase"  Biochemistry 44, 10119-10134 (2005).

Assembly members:
Kinase Interacting FHA (KI-FHA) domain from KAPP, polymer, 139 residues, 13238 Da.
CLV1 pT868 peptide, polymer, 13 residues, Formula weight is not available

Natural source:   Common Name: Thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Kinase Interacting FHA (KI-FHA) domain from KAPP: GPLGSSWLFLEVIAGPAIGL QHAVNSTSSSKLPVKLGRVS PSDLALKDSEVSGKHAQITW NSTKFKWELVDMGSLNGTLV NSHSISHPDLGSRKWGNPVE LASDDIITLGTTTKVYVRIS SQNEFQIPFKIGVASDPMA
CLV1 pT868 peptide: PEYAYTLKVDEKS

Data sets:
Data typeCount
T1 relaxation values208
T2 relaxation values212
order parameters101

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Kinase-interacting FHA domain of KAPP1
2CLV1 pT868 peptide2

Entities:

Entity 1, Kinase-interacting FHA domain of KAPP 139 residues - 13238 Da.

1   GLYPROLEUGLYSERSERTRPLEUPHELEU
2   GLUVALILEALAGLYPROALAILEGLYLEU
3   GLNHISALAVALASNSERTHRSERSERSER
4   LYSLEUPROVALLYSLEUGLYARGVALSER
5   PROSERASPLEUALALEULYSASPSERGLU
6   VALSERGLYLYSHISALAGLNILETHRTRP
7   ASNSERTHRLYSPHELYSTRPGLULEUVAL
8   ASPMETGLYSERLEUASNGLYTHRLEUVAL
9   ASNSERHISSERILESERHISPROASPLEU
10   GLYSERARGLYSTRPGLYASNPROVALGLU
11   LEUALASERASPASPILEILETHRLEUGLY
12   THRTHRTHRLYSVALTYRVALARGILESER
13   SERGLNASNGLUPHEGLNILEPROPHELYS
14   ILEGLYVALALASERASPPROMETALA

Entity 2, CLV1 pT868 peptide 13 residues - Formula weight is not available

1   PROGLUTYRALATYRTHRLEULYSVALASP
2   GLULYSSER

Samples:

sample_one: Kinase Interacting FHA (KI-FHA) domain from KAPP, [U-15N], 0.53 mM; CLV1 pT868 peptide 2.1 mM

condition_one: pH: 6.3; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
15N T1 relaxationsample_onenot availablecondition_one
15N T2 relaxationsample_onenot availablecondition_one
heteronuclear 1H-15N NOEsample_onenot availablecondition_one
1H-15N HSQCsample_onenot availablecondition_one

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Varian INOVA 600 MHz

Related Database Links:

REF NP_197429
SWISS-PROT P46014
GenBank AAK76527 AAM51227 AAB38148
PDB
BMRB 5564 5841