BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6469

Title: 1H, 13C, and 15N Chemical Shift Assignments for the HMG-box domain in the SSRP1 subunit of FACT

Deposition date: 2005-01-21 Original release date: 2005-07-25

Authors: Kasai, Nobuyuki; Tsunaka, Yasuo; Ohki, Izuru; Hirose, Susumu; Morikawa, Kosuke; Tate, Shin-ichi

Citation: Kasai, Nobuyuki; Tsunaka, Yasuo; Ohki, Izuru; Hirose, Susumu; Morikawa, Kosuke; Tate, Shin-ichi. "Solution Structure of the HMG-box domain in the SSRP1 subunit of FACT"  J. Biomol. NMR 32, 83-88 (2005).

Assembly members:
FACT-HMG, polymer, 74 residues, Formula weight is not available

Natural source:   Common Name: Fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
FACT-HMG: GSHMPKRATTAFMLWLNDTR ESIKRENPGIKVTEIAKKGG EMWKELKDKSKWEDAAAKDK QRYHDEMRNYKPEA

Data sets:
  • assigned_chemical_shifts
Data typeCount
13C chemical shifts305
1H chemical shifts533
15N chemical shifts82

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FACT-HMG polypeptide1

Entities:

Entity 1, FACT-HMG polypeptide 74 residues - Formula weight is not available

1   GLYSERHISMETPROLYSARGALATHRTHR
2   ALAPHEMETLEUTRPLEUASNASPTHRARG
3   GLUSERILELYSARGGLUASNPROGLYILE
4   LYSVALTHRGLUILEALALYSLYSGLYGLY
5   GLUMETTRPLYSGLULEULYSASPLYSSER
6   LYSTRPGLUASPALAALAALALYSASPLYS
7   GLNARGTYRHISASPGLUMETARGASNTYR
8   LYSPROGLUALA

Samples:

sample_1: FACT-HMG, [U-13C; U-15N], 2.2 mM

condition_normal: pH: 5.2; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCsample_1not availablecondition_normal
HBHA(CO)NHsample_1not availablecondition_normal
C(CO)NHsample_1not availablecondition_normal
H(CCO)NHsample_1not availablecondition_normal
HCCH-TOCSYsample_1not availablecondition_normal
HCCH-COSYsample_1not availablecondition_normal
CCH-TOCSYsample_1not availablecondition_normal
CCH-COSYsample_1not availablecondition_normal
15N-edited TOCSYsample_1not availablecondition_normal

Software:

No software information available

NMR spectrometers:

  • Bruker DMX 600 MHz
  • Bruker DMX 750 MHz

Related Database Links:

PDB
EMBL CAA48471
GB AAA28914 AAF47064 AAO45187 ACH92404 EDV56865
REF NP_523830 XP_001976465 XP_002082847 XP_002092681
SP Q05344

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts