BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6460

Title: NMR assignments of a low molecular weight phosphotyrosine phosphatase from Bacillus subtilis   PubMed: 15929004

Deposition date: 2005-01-14 Original release date: 2005-06-03

Authors: Xu, Huimin; Jin, Changwen

Citation: Xu, Huimin; Zhang, P.; Jin, Changwen. "Letter to the Editor: NMR assignments of a low molecular weight protein tyrosine phosphatase (PTPase) from Bacillus subtilis"  J. Biomol. NMR 31, 363-363 (2005).

Assembly members:
low molecular weight phosphotyrosine phosphatase, polymer, 150 residues, 16785 Da.

Natural source:   Common Name: Bacillus subtilis   Taxonomy ID: 1423   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
low molecular weight phosphotyrosine phosphatase: MDIIFVCTGNTCRSPMAEAL FKSIAEREGLNVNVRSAGVF ASPNGKATPHAVEALFEKHI ALNHVSSPLTEELMESADLV LAMTHQHKQIIASQFGRYRD KVFTLKEYVTGSHGDVLDPF GGSIDIYKQTRDELEELLRQ LAKQLKKDRR

Data sets:
Data typeCount
1H chemical shifts949
13C chemical shifts596
15N chemical shifts146

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ywlE monomer1

Entities:

Entity 1, ywlE monomer 150 residues - 16785 Da.

1   METASPILEILEPHEVALCYSTHRGLYASN
2   THRCYSARGSERPROMETALAGLUALALEU
3   PHELYSSERILEALAGLUARGGLUGLYLEU
4   ASNVALASNVALARGSERALAGLYVALPHE
5   ALASERPROASNGLYLYSALATHRPROHIS
6   ALAVALGLUALALEUPHEGLULYSHISILE
7   ALALEUASNHISVALSERSERPROLEUTHR
8   GLUGLULEUMETGLUSERALAASPLEUVAL
9   LEUALAMETTHRHISGLNHISLYSGLNILE
10   ILEALASERGLNPHEGLYARGTYRARGASP
11   LYSVALPHETHRLEULYSGLUTYRVALTHR
12   GLYSERHISGLYASPVALLEUASPPROPHE
13   GLYGLYSERILEASPILETYRLYSGLNTHR
14   ARGASPGLULEUGLUGLULEULEUARGGLN
15   LEUALALYSGLNLEULYSLYSASPARGARG

Samples:

sample_ywlE: low molecular weight phosphotyrosine phosphatase, [U-15N; U-13C], 1 mM

condition_1: pH: 7.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCOnot availablenot availablenot available
HNCAnot availablenot availablenot available
HBHA(CO)NHnot availablenot availablenot available
HCCH-COSYnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
CCH-COSYnot availablenot availablenot available
CCH-TOCSYnot availablenot availablenot available
15N-edited TOCSY-HSQCnot availablenot availablenot available
15N-edited NOESY-HSQCnot availablenot availablenot available
13C-edited NOESY-HSQCnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAI87352 BAM55776 BAM59788 GAK81006
EMBL CAA86107 CAB15710 CCU60790 CEI59501 CEJ79358
GB ADV94503 AEP92751 AFQ59544 AGA21636 AGE65300
PRF 2108403F
REF NP_391574 WP_003227664 WP_014478261 WP_014481190 WP_014906641
SP P39155

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts