BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6448

Title: Solution structure of the hypothetical protein ytfP from Escherichia coli. Northeast Structural Genomics target ER111.

Authors: Aramini, J.; Huang, Y.; Swapna, G.; Paranji, R.; Xiao, R.; Shastry, R.; Acton, T.; Cort, J.; Kennedy, M.; Montelione, G.

Citation: Aramini, J.; Swapna, G.; Huang, Y.; Rajan, P.; Xiao, R.; Shastry, R.; Acton, T.; Cort, J.; Kennedy, M.; Montelione, G.. "1H, 13C, and 15N Resonance Assignments for Escherichia coli ytfP, a Member of the Broadly Conserved UPF0131 Protein Domain Family"  J. Biomol. NMR 33, 197-197 (2005).

Assembly members:
Hypothetical UPF0131 protein ytfP, polymer, 121 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Hypothetical UPF0131 protein ytfP: MRIFVYGSLRHKQGNSHWMT NAQLLGDFSIDNYQLYSLGH YPGAVPGNGTVHGEVYRIDN ATLAELDALRTRGGEYARQL IQTPYGSAWMYVYQRPVDGL KLIESGDWLDRDKLEHHHHH H

Data sets:
Data typeCount
1H chemical shifts797
13C chemical shifts508
15N chemical shifts127
coupling constants61

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Hypothetical UPF0131 protein ytfP1

Entities:

Entity 1, Hypothetical UPF0131 protein ytfP 121 residues - Formula weight is not available

1   METARGILEPHEVALTYRGLYSERLEUARG
2   HISLYSGLNGLYASNSERHISTRPMETTHR
3   ASNALAGLNLEULEUGLYASPPHESERILE
4   ASPASNTYRGLNLEUTYRSERLEUGLYHIS
5   TYRPROGLYALAVALPROGLYASNGLYTHR
6   VALHISGLYGLUVALTYRARGILEASPASN
7   ALATHRLEUALAGLULEUASPALALEUARG
8   THRARGGLYGLYGLUTYRALAARGGLNLEU
9   ILEGLNTHRPROTYRGLYSERALATRPMET
10   TYRVALTYRGLNARGPROVALASPGLYLEU
11   LYSLEUILEGLUSERGLYASPTRPLEUASP
12   ARGASPLYSLEUGLUHISHISHISHISHIS
13   HIS

Samples:

sample_1: Hypothetical UPF0131 protein ytfP, [U-13C; U-15N], 1.3 mM; NH4OAC 20 mM; CACL2 5 mM; NaCL 0.1 M; DTT 10 mM; NAN3 0.02%; D2O 5%; H2O 95%

sample_2: Hypothetical UPF0131 protein ytfP, [U-13C; U-15N], 1.3 mM; NH4OAC 20 mM; CACL2 5 mM; NaCL 0.1 M; DTT 10 mM; NAN3 0.02%; D2O 100%

sample_3: Hypothetical UPF0131 protein ytfP, [U-5% 13C; U-15N], 1.1 mM; NH4OAC 20 mM; CACL2 5 mM; NaCL 0.1 M; DTT 10 mM; NAN3 0.02%; D2O 5%; H2O 95%

sample_cond_1: pH: 5.5; temperature: 293 K; ionic strength: 100 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 15N-SEPARATED NOESYnot availablenot availablenot available
3D 13C-SEPARATED NOESY (aliph)not availablenot availablenot available
4D 13C-SEPARATED NOESYnot availablenot availablenot available
HNHAnot availablenot availablenot available
HIGH RESOLUTION CH-HSQCnot availablenot availablenot available
3D HNCOnot availablenot availablenot available
HCCH TOCSYnot availablenot availablenot available
HCCH COSYnot availablenot availablenot available
3D 13C-SEPARATED NOESY (arom)not availablenot availablenot available
3D HNCOCACBnot availablenot availablenot available
3D HNCACBnot availablenot availablenot available
3D HNCOCAnot availablenot availablenot available
3D HNCAnot availablenot availablenot available
3D HNCACOnot availablenot availablenot available
HCCONHnot availablenot availablenot available
CCONHnot availablenot availablenot available
HACANHnot availablenot availablenot available
HACACONHnot availablenot availablenot available
HBCBcgcdHDnot availablenot availablenot available

Software:

AUTOSTRUCTURE 2.1.0, XPLOR-NIH 2.0.6, CNS v1.1 - refinement

VNMR v6.1C - structure solution

PSVS v1.0 - structure solution

NMRPIPE v2.3 - structure solution

SPARKY v3.91 - structure solution

AUTOASSIGN v1.14 - structure solution

HYPER v3.2 - structure solution

PDBSTAT v3.27 - structure solution

NMR spectrometers:

  • Varian Inova 800 MHz
  • Varian Inova 750 MHz
  • Varian Inova 600 MHz
  • Varian Inova 500 MHz

Related Database Links:

PDB
DBJ BAB38623 BAE78223 BAG80052 BAI28527 BAI33701
EMBL CAP78743 CAQ34574 CAQ91721 CAR01197 CAR05964
GB AAA97119 AAC77179 AAG59420 AAN45683 AAN83741
REF NP_290854 NP_313227 NP_418643 NP_709976 NP_757167
SP P0AE48 P0AE49 P0AE50 P0AE51