BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6446

Title: Structural and Functional Characterization of Transmembrane Segment IV of the NHE1 Isoform of the Na+/H+ Exchanger   PubMed: 15677483

Authors: Slepkov, E.; Rainey, J.; Li, X.; Liu, Y.; Cheng, F.; Lindhout, D.; Sykes, B.; Fliegel, L.

Citation: Slepkov, E.; Rainey, J.; Li, X.; Liu, Y.; Cheng, F.; Lindhout, D.; Sykes, B.; Fliegel, L.. "The Na+/H+ Exchanger isoform 1"  J. Biol. Chem. 280, 17863-17872 (2005).

Assembly members:
Sodium/hydrogen exchanger 1, polymer, 27 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Sodium/hydrogen exchanger 1: FLQSDVFFLFLLPPIILDAG YFLPLRX

Data sets:
Data typeCount
1H chemical shifts135
13C chemical shifts52
15N chemical shifts25

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Sodium/hydrogen exchanger 1 TM segment IV1

Entities:

Entity 1, Sodium/hydrogen exchanger 1 TM segment IV 27 residues - Formula weight is not available

1   PHELEUGLNSERASPVALPHEPHELEUPHE
2   LEULEUPROPROILEILELEUASPALAGLY
3   TYRPHELEUPROLEUARGHSL

Samples:

sample_1: Sodium/hydrogen exchanger 1 2 mM; DSS 1 mM; CDCl3 44%; CD3OH 44%; H2O 11%

sample_2: Sodium/hydrogen exchanger 1, [U-15N], 2 mM; DSS 1 mM; CDCl3 44%; CD3OH 44%; H2O 11%

sample_cond_1: pH: .; temperature: 303 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
DQF-COSYnot availablenot availablesample_cond_1
HNHAnot availablenot availablesample_cond_1
2D NOESYnot availablenot availablesample_cond_1
2D TOCSYnot availablenot availablesample_cond_1

Software:

VNMR vvarious - collection

NMRPipe v2.3 - processing

SPARKY v3.109 and 3.110 - data analysis

CNS v1.1 - structure solution, refinement

NMR spectrometers:

  • Varian INOVA 500 MHz

Related Database Links:

PDB
DBJ BAE02258 BAE29332 BAE32882 BAE38221 BAG36869
EMBL CAA48771
GB AAA92976 AAA98479 AAB59460 AAC60606 AAF21350
REF NP_001233682 NP_001273957 NP_003038 NP_036784 NP_058677
SP P19634 P26431 P48761 Q61165