BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6444

Title: 1H, 13C, and 15N Chemical Shift Assignments for MMP12

Authors: Bertini, Ivano; Calderone, Vito; Cosenza, Marta; Fragai, Marco; Lee, Yong-Min; Luchinat, Claudio; Mangani, Stefano; Terni, Beatrice; Turano, Paola

Citation: Bertini, Ivano; Calderone, Vito; Cosenza, Marta; Fragai, Marco; Lee, Yong-Min; Luchinat, Claudio; Mangani, Stefano; Terni, Beatrice; Turano, Paola. "Conformational Variability of MMPs: Beyond a Single 3D Structure"  Proc. Natl. Acad. Sci. U.S.A. 102, 5334-5339 (2005).

Assembly members:
matrix metalloelastase, polymer, 159 residues, Formula weight is not available
NGH, non-polymer, 316.373 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
matrix metalloelastase: MGPVWRKHYITYRINNYTPD MNREDVDYAIRKAFQVWSNV TPLKFSKINTGMADILVVFA RGAHGDDHAFDGKGGILAHA FGPGSGIGGDAHFDEDEFWT THSGGTNLFLTAVHEIGHSL GLGHSSDPKAVMFPTYKYVD INTFRLSADDIRGIQSLYG

Data sets:
Data typeCount
1H chemical shifts909
13C chemical shifts318
15N chemical shifts157
residual dipolar couplings111

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MMP12 catalytic domain1
2NNGH2

Entities:

Entity 1, MMP12 catalytic domain 159 residues - Formula weight is not available

1   METGLYPROVALTRPARGLYSHISTYRILE
2   THRTYRARGILEASNASNTYRTHRPROASP
3   METASNARGGLUASPVALASPTYRALAILE
4   ARGLYSALAPHEGLNVALTRPSERASNVAL
5   THRPROLEULYSPHESERLYSILEASNTHR
6   GLYMETALAASPILELEUVALVALPHEALA
7   ARGGLYALAHISGLYASPASPHISALAPHE
8   ASPGLYLYSGLYGLYILELEUALAHISALA
9   PHEGLYPROGLYSERGLYILEGLYGLYASP
10   ALAHISPHEASPGLUASPGLUPHETRPTHR
11   THRHISSERGLYGLYTHRASNLEUPHELEU
12   THRALAVALHISGLUILEGLYHISSERLEU
13   GLYLEUGLYHISSERSERASPPROLYSALA
14   VALMETPHEPROTHRTYRLYSTYRVALASP
15   ILEASNTHRPHEARGLEUSERALAASPASP
16   ILEARGGLYILEGLNSERLEUTYRGLY

Entity 2, NNGH - C13 H20 N2 O5 S - 316.373 Da.

1   NGH

Samples:

sample_1: matrix metalloelastase, [U-13C; U-15N], 0.9 mM; N-Isobutyl-N-[4-methoxyphenylsulfonyl]glycyl hydroxamic acid 0.9 mM

sample_2: matrix metalloelastase, [U-13C; U-15N], 0.9 mM; N-Isobutyl-N-[4-methoxyphenylsulfonyl]glycyl hydroxamic acid 0.9 mM

conditions_1: pH: 7.20; temperature: 298.0 K

Experiments:

NameSampleSample stateSample conditions
3D 13N-separated NOESYsample_1not availableconditions_1
2D NOESYsample_1not availableconditions_1
one bond 1H-15N IPAP HSQCsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker AVANCE 900 MHz
  • Bruker AVANCE 800 MHz
  • Bruker AVANCE 700 MHz
  • Bruker DRX 500 MHz

Related Database Links:

REF XP_508724 NP_002417
SP P39900
GB EAW67033 AAI43774 AAW29944 AAA58658 AAI12302
PDB
DBJ BAG36675
BMRB 7089 7415 15578 6391