BMRB Entry 6415

Title:
Three-disulfide variant of hen lysozyme: C30A/C115A
Deposition date:
2004-12-07
Original release date:
2005-04-04
Authors:
Yokota, Atsushi; Hirai, Kenichi; Miyauchi, Hiroyo; Iimura, Satoshi; Noda, Yasuo; Inoue, Kyouko; Akasaka, Kazuyuki; Tachibana, Hideki; Segawa, Shin-ichi
Citation:

Citation: Yokota, Atsushi; Hirai, Kenichi; Miyauchi, Hiroyo; Iimura, Satoshi; Noda, Yasuo; Inoue, Kyouko; Akasaka, Kazuyuki; Tachibana, Hideki; Segawa, Shin-ichi. "NMR Characterization of Three-Disulfide Variants of Lysozyme, C64A/C80A, C76A/C94A, and C30A/C115A -A Marginally Stable State in Folded Proteins"  Biochemistry 43, 6663-6669 (2004).
PubMed: 15157100

Assembly members:

Assembly members:
hen egg white lysozyme variant, polymer, 130 residues, 14374 Da.

Natural source:

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Data sets:
Data typeCount
1H chemical shifts752

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hen lysozyme1

Entities:

Entity 1, hen lysozyme 130 residues - 14374 Da.

1   METLYSVALPHEGLYARGCYSGLULEUALA
2   ALAALAMETLYSARGHISGLYLEUASPASN
3   TYRARGGLYTYRSERLEUGLYASNTRPVAL
4   ALAALAALALYSPHEGLUSERASNPHEASN
5   THRGLNALATHRASNARGASNTHRASPGLY
6   SERTHRASPTYRGLYILELEUGLNILEASN
7   SERARGTRPTRPCYSASNASPGLYARGTHR
8   PROGLYSERARGASNLEUCYSASNILEPRO
9   CYSSERALALEULEUSERSERASPILETHR
10   ALASERVALASNCYSALALYSLYSILEVAL
11   SERASPGLYASNGLYMETASNALATRPVAL
12   ALATRPARGASNARGALALYSGLYTHRASP
13   VALGLNALATRPILEARGGLYCYSARGLEU

Samples:

sample_1: hen egg white lysozyme variant, [U-15N], 0.2 – 1.0 mM

EX-cond_1: pH: 3.8 na; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
DQF-COSYsample_1not availableEX-cond_1
NOESYsample_1not availableEX-cond_1
HOHAHAsample_1not availableEX-cond_1
15N 3D-NOESY-HSQCsample_1not availableEX-cond_1
15N 3D-TOCSY-HSQCsample_1not availableEX-cond_1
1H-15N HSQCsample_1not availableEX-cond_1
15N 3D-HSQC-NOESY-HSQCsample_1not availableEX-cond_1

Software:

XWINNMR v2.6 -

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

GenBank AAL69327 AAA48943
SWISS-PROT P00698
PDB
BMRB 4943 5068 1093 4562