BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6368

Title: 1H, 13C, and 15N Chemical Shift Assignments of Methanosarcina mazei Protein RPS24E: The Northeast Structural Genomics Consortium Target MaR11   PubMed: 16027363

Deposition date: 2004-10-27 Original release date: 2008-08-28

Authors: Liu, Gaohua; Xiao, Rong; Parish, David; Ma, LiChung; Sukumaran, Dinesh; Acton, Thomas; Montelione, Gaetano; Szyperski, Thomas

Citation: Liu, Gaohua; Shen, Yang; Atreya, Hanudatta; Parish, David; Shao, Ying; Sukumaran, Dinesh; Xiao, Rong; Yee, Adelinda; Lemak, Alexander; Bhattacharya, Aneerban; Acton, Thomas; Arrowsmith, Cheryl; Montelione, Gaetano; Szyperski, Thomas. "NMR data collection and analysis protocol for high-throughput protein structure determination"  Proc. Natl. Acad. Sci. U.S.A. 102, 10487-10492 (2005).

Assembly members:
Hypothetical protein rps24e, polymer, 101 residues, Formula weight is not available

Natural source:   Common Name: Methanosarcina mazei   Taxonomy ID: 2209   Superkingdom: Archaea   Kingdom: Not applicable   Genus/species: Methanosarcina mazei

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Hypothetical protein rps24e: MDIKIIKDKKNPLLNRRELD FIVKYEGSTPSRNDVRNKLA AMLNAPLELLVIQRIKTEYG MQESKGYAKLYEDADRMKQV EQEYVLKRNAVPGSETEGEE A

Data sets:
Data typeCount
1H chemical shifts763
13C chemical shifts364
15N chemical shifts113

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Hypothetical protein rps24e1

Entities:

Entity 1, Hypothetical protein rps24e 101 residues - Formula weight is not available

1   METASPILELYSILEILELYSASPLYSLYS
2   ASNPROLEULEUASNARGARGGLULEUASP
3   PHEILEVALLYSTYRGLUGLYSERTHRPRO
4   SERARGASNASPVALARGASNLYSLEUALA
5   ALAMETLEUASNALAPROLEUGLULEULEU
6   VALILEGLNARGILELYSTHRGLUTYRGLY
7   METGLNGLUSERLYSGLYTYRALALYSLEU
8   TYRGLUASPALAASPARGMETLYSGLNVAL
9   GLUGLNGLUTYRVALLEULYSARGASNALA
10   VALPROGLYSERGLUTHRGLUGLYGLUGLU
11   ALA

Samples:

sample_1: Hypothetical protein rps24e, [U-13C; U-15N], 1.0 mM; MES 20 mM; NaCl 100 mM; DTT 10 mM; CaCl2 5 mM; NaN3 0.02%; H2O 95%; D2O 5%

sample_conditions: temperature: 298 K; pH: 6.5; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
GFT (4,3)D HNNCabCanot availablenot availablenot available
GFT (4,3)D CabCa(CO)NHNnot availablenot availablenot available
GFT (4,3)D HabCab(CO)NHNnot availablenot availablenot available
GFT (4,3)D HCCHnot availablenot availablenot available
SIMULTANEOUS HETERONUCLEAR RESOLVED [1H,1H]-NOESYnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 750 MHz

Related Database Links:

PDB
GB AAM30295 AGF96024
REF NP_632623 WP_011032550 WP_015411211 YP_007489296
SP Q8PZ95

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts