BMRB Entry 6364

Title:
1H, 13C 15N chemical shift assignment of Pyrococcus furiosus Protein PF0470: The Northeast Structural Genomics Consortium Target PfR14
Deposition date:
2004-10-27
Original release date:
2008-08-28
Authors:
Liu, Gaohua; Xiao, Rong; Parish, David; Ma, Lichung; Sukumaran, Dinesh; Acton, Thomas; Montelione, Gaetano; Szyperski, Thomas
Citation:

Citation: Liu, Gaohua; Shen, Yang; Atreya, Hanudatta; Parish, David; Shao, Ying; Sukumaran, Dinesh; Xiao, Rong; Yee, Adelinda; Lemak, Alexander; Bhattacharya, Aneerban; Acton, Thomas; Arrowsmith, Cheryl; Montelione, Gaetano; Szyperski, Thomas. "NMR data collection and analysis protocol for high-throughput protein structure determination"  Proc. Natl. Acad. Sci. U.S.A. 102, 10487-10492 (2005).
PubMed: 16027363

Assembly members:

Assembly members:
Protein PF0470, polymer, 113 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Pyrococcus furiosus   Taxonomy ID: 2261   Superkingdom: Archaea   Kingdom: not available   Genus/species: Pyrococcus furiosus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Data sets:
Data typeCount
1H chemical shifts815
13C chemical shifts415
15N chemical shifts90

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Protein PF04701

Entities:

Entity 1, Protein PF0470 113 residues - Formula weight is not available

1   METLYSVALTYRARGLEUTYRLEULYSASP
2   GLUTYRLEUGLUMETVALLYSSERGLYLYS
3   LYSARGILEGLUVALARGVALALATYRPRO
4   GLNLEULYSASPILELYSARGGLYASPLYS
5   ILEILEPHEASNASPLEUILEPROALAGLU
6   VALVALGLUVALLYSLYSTYRGLUTHRPHE
7   ARGGLNVALLEUARGGLUGLUPROILEASP
8   LYSILEPHEPROASPLYSPROSERPHEGLU
9   LYSALALEULYSARGPHEHISASNMETTYR
10   PROLYSTRPLYSGLUTYRARGTYRGLYVAL
11   LEUALAILELYSPHEARGVALLEUGLYARG
12   ASPLYSGLU

Samples:

sample: Protein PF0470, [U-13C; U-15C], 1 mM; MES 20 mM; NaCl 100 mM; DTT 10 mM; CaCl2 5 mM; NaN3 0.02%; H2O 95%; D2O 5%

sample_conditions: temperature: 298 K; pH: 6.5; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
GFT (4,3)D HNNCabCa'not availablenot availablenot available
GFT (4,3)D CabCa(CO)NHN'not availablenot availablenot available
GFT (4,3)D HabCab(CO)NHN'not availablenot availablenot available
GFT (4,3) HCCH'not availablenot availablenot available
Simultaneous Heteronuclear Resolved NOESYnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Varian Inova 600 MHz
  • Varian Inova 750 MHz

Related Database Links:

PDB
GB AAL80594 AFN03264
REF NP_578199 WP_011011587 YP_006491556

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks