BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6332

Title: 1H, 13C and 15N resonance assignments of MLC (myosin light chain)from Saccharomyces cerevisiae

Authors: Pennestri, Matteo; Cicero, Daniel

Citation: Melino, Sonia; Pennestri, Matteo; Santoprete, Alessia; Bielli, Pamela; Paci, Maurizio; Ragnini-Wilson, Antonella; Cicero, Daniel. "Letter to the Editor: Assignment of the 1H, 13C and 15N resonances of Mlc1p from Saccharomyces cerevisiae"  J. Biomol. NMR 31, 367-368 (2005).

Assembly members:
Myosin Light Chain, polymer, 149 residues, Formula weight is not available

Natural source:   Common Name: baker   Taxonomy ID: 4932   Superkingdom: not available   Kingdom: not available   Genus/species: Eukaryota Fungi

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Myosin Light Chain: MSATRANKDIFTLFDKKGQG AIAKDSLGDYLRAIGYNPTN QLVQDIINADSSLRDASSLT LDQITGLIEVNEKELDATTK AKTEDFVKAFQVFDKESTGK VSVGDLRYMLTGLGEKLTDA EVDELLKGVEVDSNGEIDYK KFIEDVLRQ

Data sets:
Data typeCount
1H chemical shifts1010
13C chemical shifts664
15N chemical shifts155
T1 relaxation values125
T2 relaxation values125

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1mlc monomer1

Entities:

Entity 1, mlc monomer 149 residues - Formula weight is not available

1   METSERALATHRARGALAASNLYSASPILE
2   PHETHRLEUPHEASPLYSLYSGLYGLNGLY
3   ALAILEALALYSASPSERLEUGLYASPTYR
4   LEUARGALAILEGLYTYRASNPROTHRASN
5   GLNLEUVALGLNASPILEILEASNALAASP
6   SERSERLEUARGASPALASERSERLEUTHR
7   LEUASPGLNILETHRGLYLEUILEGLUVAL
8   ASNGLULYSGLULEUASPALATHRTHRLYS
9   ALALYSTHRGLUASPPHEVALLYSALAPHE
10   GLNVALPHEASPLYSGLUSERTHRGLYLYS
11   VALSERVALGLYASPLEUARGTYRMETLEU
12   THRGLYLEUGLYGLULYSLEUTHRASPALA
13   GLUVALASPGLULEULEULYSGLYVALGLU
14   VALASPSERASNGLYGLUILEASPTYRLYS
15   LYSPHEILEGLUASPVALLEUARGGLN

Samples:

sample_mlc: Myosin Light Chain, [U-99% 13C; U-99% 15N], 0.7 mM; phosphate buffer 66 mM; NaCl 0.33 mM

Ex-cond: pH: 6.9; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
1H-15N TOCSYnot availablenot availablenot available
1H-15N NOESYnot availablenot availablenot available
CBCACONHnot availablenot availablenot available
CBCANHnot availablenot availablenot available
HBHACONHnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCOCAnot availablenot availablenot available
HNCOnot availablenot availablenot available
hacaCONHnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

PDB
DBJ GAA23287
EMBL CAA66246 CAA96813 CAY79658
GB AHY79275 EDN62013 EDV10328 EDZ72226 EEU08150
REF NP_011409
SP P53141
TPG DAA08001