BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6331

Title: NMR assignment of the SH2 domain from the human feline sarcoma oncogene FES

Authors: Scott, Anna; Pantoja-Uceda, David; Koshiba, Seizo; Inoue, Makoto; Kigawa, Takanori; Terada, Takaho; Shirouzu, Mikako; Tanaka, Akiko; Sugano, Sumio; Yokoyama, Shigeyuki; Guntert, Peter

Citation: Scott, Anna; Pantoja-Uceda, David; Koshiba, Seizo; Inoue, Makoto; Kigawa, Takanori; Terada, Takaho; Shirouzu, Mikako; Tanaka, Akiko; Sugano, Sumio; Yokoyama, Shigeyuki; Guntert, Peter. "Letter to the Editor: NMR assignment of the SH2 domain from the human feline sarcoma oncogene FES"  J. Biomol. NMR 30, 463-464 (2004).

Assembly members:
SH2 domain from the human feline sarcoma oncogene FES, polymer, 114 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
SH2 domain from the human feline sarcoma oncogene FES: GSSGSSGEVQKPLHEQLWYH GAIPRAEVAELLVHSGDFLV RESQGKQEYVLSVLWDGLPR HFIIQSLDNLYRLEGEGFPS IPLLIDHLLSTQQPLTKKSG VVLHRAVPSGPSSG

Data sets:
Data typeCount
13C chemical shifts492
1H chemical shifts797
15N chemical shifts111

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SH2 domain1

Entities:

Entity 1, SH2 domain 114 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYGLUVALGLN
2   LYSPROLEUHISGLUGLNLEUTRPTYRHIS
3   GLYALAILEPROARGALAGLUVALALAGLU
4   LEULEUVALHISSERGLYASPPHELEUVAL
5   ARGGLUSERGLNGLYLYSGLNGLUTYRVAL
6   LEUSERVALLEUTRPASPGLYLEUPROARG
7   HISPHEILEILEGLNSERLEUASPASNLEU
8   TYRARGLEUGLUGLYGLUGLYPHEPROSER
9   ILEPROLEULEUILEASPHISLEULEUSER
10   THRGLNGLNPROLEUTHRLYSLYSSERGLY
11   VALVALLEUHISARGALAVALPROSERGLY
12   PROSERSERGLY

Samples:

sample_1: SH2 domain from the human feline sarcoma oncogene FES, [U-13C; U-15N], 1.2 mM

sample_conditions: pH: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
15N HSQCsample_1not availablesample_conditions
15N NOESY-HSQCsample_1not availablesample_conditions
15N TOCSY-HSQCsample_1not availablesample_conditions
13C HSQCsample_1not availablesample_conditions
13C NOESY-HSQCsample_1not availablesample_conditions
HCCH-TOCSYsample_1not availablesample_conditions
HCCH-COSYsample_1not availablesample_conditions
HNCOsample_1not availablesample_conditions
HN(CO)CAsample_1not availablesample_conditions
HNCAsample_1not availablesample_conditions
CBCA(CO)NHsample_1not availablesample_conditions
CBCANHsample_1not availablesample_conditions
HBHA(CO)NHsample_1not availablesample_conditions
(H)CC(CO)NHsample_1not availablesample_conditions
H(CCCO)NHsample_1not availablesample_conditions

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker AV 800 MHz

Related Database Links:

PDB
DBJ BAG35443 BAG62301 BAJ20714
EMBL CAA29619 CAA36438
GB AAH35357 AAS82868 AAX29908 AAX42468 ABM82276
REF NP_001137255 NP_001996 XP_001093848 XP_001169472 XP_003268554
SP P07332