BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6330

Title: Solution structure of human AP4A hydrolase

Authors: Swarbrick, James

Citation: Swarbrick, James; Buyya, S.; Gunawardana, D.; Fletcher, Jamie; Branson, Kim; Smith, Brian; Pepe, Salvatore; McLennan, A.; Gayler, K.; Gooley, Paul. "Letter to the Editor: 1H, 13C, and 15N resonance assignments of the 17 kDa Ap4A hydrolase from Homo sapiens in the presence and absence of ATP"  J. Biomol. NMR 31, 181-182 (2005).

Assembly members:
AP4A, polymer, 153 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
AP4A: GPLGSMALRACGLIIFRRCL IPKVDNNAIEFLLLQASDGI HHWTPPKGHVEPGEDDLETA LRATQEEAGIEAGQLTIIEG FKRELNYVARNKPKTVIYWL AEVKDYDVEIRLSHEHQAYR WLGLEEACQLAQFKEMKAAL QEGHQFLCSIEAL

Data sets:
Data typeCount
13C chemical shifts617
1H chemical shifts1046
15N chemical shifts147

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AP4A hydrolase monomer1

Entities:

Entity 1, AP4A hydrolase monomer 153 residues - Formula weight is not available

1   GLYPROLEUGLYSERMETALALEUARGALA
2   CYSGLYLEUILEILEPHEARGARGCYSLEU
3   ILEPROLYSVALASPASNASNALAILEGLU
4   PHELEULEULEUGLNALASERASPGLYILE
5   HISHISTRPTHRPROPROLYSGLYHISVAL
6   GLUPROGLYGLUASPASPLEUGLUTHRALA
7   LEUARGALATHRGLNGLUGLUALAGLYILE
8   GLUALAGLYGLNLEUTHRILEILEGLUGLY
9   PHELYSARGGLULEUASNTYRVALALAARG
10   ASNLYSPROLYSTHRVALILETYRTRPLEU
11   ALAGLUVALLYSASPTYRASPVALGLUILE
12   ARGLEUSERHISGLUHISGLNALATYRARG
13   TRPLEUGLYLEUGLUGLUALACYSGLNLEU
14   ALAGLNPHELYSGLUMETLYSALAALALEU
15   GLNGLUGLYHISGLNPHELEUCYSSERILE
16   GLUALALEU

Samples:

sample_1: AP4A mM; MgCl2 20 mM; Imidazole 20 mM; H2o 90%; D2o 10%

sample_conditions: pH: 6.5; temperature: 293 K; ionic strength: 20 mM

Experiments:

NameSampleSample stateSample conditions
not availablesample_1not availablesample_conditions

Software:

nmrPipe v2.1, F. Delaglio -

XEASY v1.4, Kurt Wuthrich -

NMR spectrometers:

  • Varian Inova 600 MHz
  • Varian Inova 500 MHz

Related Database Links:

BMRB 6336
PDB
GB AAC50277 AAH04926 AAX36304 AAX36755 AAX41136
REF NP_001152 NP_001231319 NP_671701 NP_671702 XP_001160435
SP P50583