BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6319

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Nkx2.5 Homeodomain plus NK2 Specific Domain in the DNA Bound State

Authors: Gruschus, James; Lee, Hyun-Sook; Ferretti, James

Citation: Lee, Hyun-Sook; Gruschus, James; Zhang, Tao; Ferretti, James. "Letter to the Editor: NMR assignments of the DNA-bound human Csx/Nkx2.5 homeodomain and NK2-specific domain"  J. Biomol. NMR 31, 75-76 (2005).

Assembly members:
nkx2.5 homeodomain plus NK2 specific domain, polymer, 152 residues, Formula weight is not available
DNA, polymer, 16 residues, Formula weight is not available
DNA, polymer, 16 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Animalia   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
nkx2.5 homeodomain plus NK2 specific domain: MHHHHHHSSGLVPRGSGMKE TAAAKFERQHMDSPDLGTDD DDKAMAERPRARRRRKPRVL FSQAQVYELERRFKQQRYLS APERDQLASVLKLTSTQVKI WFQNRRYKCKRQRQDQTLEL VGLPPPPPPPARRIAVPVLV RDGKPCLGDSAP
DNA: TGTGTCAAGTGGCTGT
DNA: ACAGCCACTTGACACA

Data sets:
Data typeCount
1H chemical shifts255
13C chemical shifts169
15N chemical shifts134

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1nkx2.51
2DNA strand 12
3DNA strand 23

Entities:

Entity 1, nkx2.5 152 residues - Formula weight is not available

1   METHISHISHISHISHISHISSERSERGLY
2   LEUVALPROARGGLYSERGLYMETLYSGLU
3   THRALAALAALALYSPHEGLUARGGLNHIS
4   METASPSERPROASPLEUGLYTHRASPASP
5   ASPASPLYSALAMETALAGLUARGPROARG
6   ALAARGARGARGARGLYSPROARGVALLEU
7   PHESERGLNALAGLNVALTYRGLULEUGLU
8   ARGARGPHELYSGLNGLNARGTYRLEUSER
9   ALAPROGLUARGASPGLNLEUALASERVAL
10   LEULYSLEUTHRSERTHRGLNVALLYSILE
11   TRPPHEGLNASNARGARGTYRLYSCYSLYS
12   ARGGLNARGGLNASPGLNTHRLEUGLULEU
13   VALGLYLEUPROPROPROPROPROPROPRO
14   ALAARGARGILEALAVALPROVALLEUVAL
15   ARGASPGLYLYSPROCYSLEUGLYASPSER
16   ALAPRO

Entity 2, DNA strand 1 16 residues - Formula weight is not available

1   DTDGDTDGDTDCDADADGDT
2   DGDGDCDTDGDT

Entity 3, DNA strand 2 16 residues - Formula weight is not available

1   DADCDADGDCDCDADCDTDT
2   DGDADCDADCDA

Samples:

sample: nkx2.5 homeodomain plus NK2 specific domain, [U-13C; U-15N], 0.4 mM

sample_conditions: pH: 5; temperature: 307 K

Experiments:

NameSampleSample stateSample conditions
not availablesamplenot availablesample_conditions

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

GB AAI14090 AAS99114 ABB00236 ABC71311 EHB08399
REF NP_001010959 NP_001039908 XP_001096796 XP_002710431 XP_002744612
TPG DAA18041