BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6255

Title: Chemical Shift Assignments of Ribosomal Protein L16 from Thermus thermophilus HB8

Authors: Nishimura, Mitsuhiro; Yoshida, Takuya; Shirouzu, Mikako; Terada, Takaho; Kuramitsu, Seiki; Yokoyama, Shigeyuki; Ohkubo, Tadayasu; Kobayashi, Yuji

Citation: Nishimura, Mitsuhiro; Yoshida, Takuya; Shirouzu, Mikako; Terada, Takaho; Kuramitsu, Seiki; Yokoyama, Shigeyuki; Ohkubo, Tadayasu; Kobayashi, Yuji. "Solution Structure of Ribosomal Protein L16 from Thermus thermophilus HB8"  J. Mol. Biol. 344, 1369-1383 (2004).

Assembly members:
Ribosomal Protein L16, polymer, 141 residues, Formula weight is not available

Natural source:   Common Name: Thermus thermophilus   Taxonomy ID: 274   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Thermus thermohilus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Ribosomal Protein L16: MLMPRRMKYRKQQRGRLKGA TKGGDYVAFGDYGLVALEPA WITAQQIEAARVAMVRHFRR GGKIFIRIFPDKPYTKKPLE VRMGKGKGNVEGYVAVVKPG RVMFEVAGVTEEQAMEALRI AGHKLPIKTKIVRRDAYDEA Q

Data sets:
Data typeCount
1H chemical shifts960
13C chemical shifts624
15N chemical shifts134

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ribosomal Protein L161

Entities:

Entity 1, Ribosomal Protein L16 141 residues - Formula weight is not available

1   METLEUMETPROARGARGMETLYSTYRARG
2   LYSGLNGLNARGGLYARGLEULYSGLYALA
3   THRLYSGLYGLYASPTYRVALALAPHEGLY
4   ASPTYRGLYLEUVALALALEUGLUPROALA
5   TRPILETHRALAGLNGLNILEGLUALAALA
6   ARGVALALAMETVALARGHISPHEARGARG
7   GLYGLYLYSILEPHEILEARGILEPHEPRO
8   ASPLYSPROTYRTHRLYSLYSPROLEUGLU
9   VALARGMETGLYLYSGLYLYSGLYASNVAL
10   GLUGLYTYRVALALAVALVALLYSPROGLY
11   ARGVALMETPHEGLUVALALAGLYVALTHR
12   GLUGLUGLNALAMETGLUALALEUARGILE
13   ALAGLYHISLYSLEUPROILELYSTHRLYS
14   ILEVALARGARGASPALATYRASPGLUALA
15   GLN

Samples:

sample_1: Ribosomal Protein L16, [U-15N], 0.6 mM; Sodium acetate 10 mM; Sodium chloride 100 mM

sample_2: Ribosomal Protein L16, [U-13C; U-15N], 0.6 mM; Sodium acetate 10 mM; Sodium chloride 100 mM

sample_cond: pH: 5.4 na; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H15N HSQCnot availablenot availablesample_cond
15N-separated NOESYnot availablenot availablesample_cond
CBCA(CO)NHnot availablenot availablesample_cond
CBCANHnot availablenot availablesample_cond
HNCAHAnot availablenot availablesample_cond
C(CO)NHnot availablenot availablesample_cond
HCCH-TOCSYnot availablenot availablesample_cond
HBHA(CO)NHnot availablenot availablesample_cond
H(CCO)NHnot availablenot availablesample_cond
HNCOnot availablenot availablesample_cond
13C-1H HSQCnot availablenot availablesample_cond
13C-separated NOESYnot availablenot availablesample_cond

Software:

NMRPipe - Data processing

SPARKY v3.106, Spectrum analysis - Data processing

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAD71508
GB AAS81663 AEG34098 AFH38269 EED11193 EIA38546
REF WP_003043874 WP_008633417 WP_011173709 WP_011228846 WP_019550491
SP P60489 Q72I11