BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6243

Title: NMR Relaxation data for Protein Azurin from Pseudomonas aeruginosa   PubMed: 15364584

Authors: Zhuravleva, Anastasia; Korzhnev, Dmitry; Kupce, Eriks; Billeter, Martin; Arseniev, Alexander; Orekhov, Vladislav

Citation: Zhuravleva, Anastasia; Korzhnev, Dmitry; Kupce, Eriks; Arseniev, Alexander; Billeter, Martin; Orekhov, Vladislav. "Gated electron transfers and electron pathways in azurin: a NMR dynamic study at multiple fields and temperatures"  J. Mol. Biol. 342, 1599-1611 (2004).

Assembly members:
azurin, polymer, 128 residues, Formula weight is not available
CU1, non-polymer, 63.546 Da.

Natural source:   Common Name: Pseudomonas aeruginosa   Taxonomy ID: 287   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
azurin: AECSVDIQGNDQMQFNTNAI TVDKSCKQFTVNLSHPGNLP KNVMGHNWVLSTAADMQGVV TDGMASGLDKDYLKPDDSRV IAHTKLIGSGEKDSVTFDVS KLKEGEQYMFFCTFPGHSAL MKGTLTLK

Data typeCount
order parameters230
T1 relaxation values806
T2 relaxation values806

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1azurin1
2COPPER (I) ION2

Entities:

Entity 1, azurin 128 residues - Formula weight is not available

1   ALAGLUCYSSERVALASPILEGLNGLYASN
2   ASPGLNMETGLNPHEASNTHRASNALAILE
3   THRVALASPLYSSERCYSLYSGLNPHETHR
4   VALASNLEUSERHISPROGLYASNLEUPRO
5   LYSASNVALMETGLYHISASNTRPVALLEU
6   SERTHRALAALAASPMETGLNGLYVALVAL
7   THRASPGLYMETALASERGLYLEUASPLYS
8   ASPTYRLEULYSPROASPASPSERARGVAL
9   ILEALAHISTHRLYSLEUILEGLYSERGLY
10   GLULYSASPSERVALTHRPHEASPVALSER
11   LYSLEULYSGLUGLYGLUGLNTYRMETPHE
12   PHECYSTHRPHEPROGLYHISSERALALEU
13   METLYSGLYTHRLEUTHRLEULYS

Entity 2, COPPER (I) ION - Cu - 63.546 Da.

1   CU1

Samples:

sample_1: azurin, [U-95% 15N], 1 – 1 mM

condition_one: pH: 5.5; temperature: 289 K

condition_two: pH: 5.5; temperature: 317 K

Experiments:

NameSampleSample stateSample conditions
15N-1H HSQCsample_1not availablenot available
15N T2sample_1not availablenot available
1H-15N NOEsample_1not availablenot available

Software:

NMRPipe -

DASHA -

MUNIN -

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz
  • Varian INOVA 900 MHz

Related Database Links:

BMRB 1210 1211 1212 1213 1214 1215 1216 1217 1218 1219 18254 4465
PDB
DBJ BAK87490 BAP24727 BAP53499 GAA20615
EMBL CAA30279 CAW30062 CCQ86145 CDH73668 CDH79986
GB AAA25730 AAG08307 AAP03090 AAT49489 ABJ14307
PRF 671048A
REF NP_253609 WP_003095591 WP_003141697 WP_012077620 WP_031690945
SP B3EWN9 P00282