BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6240

Title: Solution structure of At5g66040, a putative protein from Arabidopsis Thaliana

Authors: Cornilescu, Claudia; Cornilescu, Gabriel; Singh, Shanteri; Lee, Min; Tyler, Ejan; Shahan, Mark; Vinarov, Dmitriy; Markley, John

Citation: Cornilescu, Claudia; Cornilescu, Gabriel; Singh, Shanteri; Lee, Min; Tyler, Ejan; Shahan, Mark; Vinarov, Dmitriy; Markley, John. "Solution structure of At5g66040, a putative protein from Arabidopsis Thaliana"  J. Biomol. NMR ., .-..

Assembly members:
At5g66040, polymer, 129 residues, 13802.3 Da.

Natural source:   Common Name: thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
At5g66040: MGHHHHHHLEAEESRVPSSV SVTVAHDLLLAGHRYLDVRT PEEFSQGHACGAINVPYMNR GASGMSKNTDFLEQVSSHFG QSDNIIVGCQSGGRSIKATT DLLHAGFTGVKDIVGGYSAW AKNGLPTKA

Data sets:
Data typeCount
1H chemical shifts614
13C chemical shifts413
15N chemical shifts106

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1At5g660401

Entities:

Entity 1, At5g66040 129 residues - 13802.3 Da.

1   METGLYHISHISHISHISHISHISLEUGLU
2   ALAGLUGLUSERARGVALPROSERSERVAL
3   SERVALTHRVALALAHISASPLEULEULEU
4   ALAGLYHISARGTYRLEUASPVALARGTHR
5   PROGLUGLUPHESERGLNGLYHISALACYS
6   GLYALAILEASNVALPROTYRMETASNARG
7   GLYALASERGLYMETSERLYSASNTHRASP
8   PHELEUGLUGLNVALSERSERHISPHEGLY
9   GLNSERASPASNILEILEVALGLYCYSGLN
10   SERGLYGLYARGSERILELYSALATHRTHR
11   ASPLEULEUHISALAGLYPHETHRGLYVAL
12   LYSASPILEVALGLYGLYTYRSERALATRP
13   ALALYSASNGLYLEUPROTHRLYSALA

Samples:

sample_1: At5g66040, [U-13C; U-15N], 1 mM

sample_cond: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
15N-HSQCnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCACONHnot availablenot availablenot available
HNCOnot availablenot availablenot available
HBHACONHnot availablenot availablenot available
HCCONHnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
3D-15N NOESYnot availablenot availablenot available
3D-13C NOESYnot availablenot availablenot available
15N-T2not availablenot availablenot available

Software:

nmrPipe - data processing, automated peak picking

PIPP/STAPP - manual peak assignments

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Bruker AVANCE 750 MHz
  • Bruker AVANCE 500 MHz

Related Database Links:

PDB
DBJ BAB10409
GB AAK83644 AAM61332 AAN38701 AED98145 AED98146
REF NP_569026 NP_851278
SP Q39129