BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6234

Title: Chemical shifts of PagP in CYFOS-7 detergent

Authors: Hwang, Peter; Bishop, Russell; Kay, Lewis

Citation: Hwang, Peter; Bishop, Russell; Kay, Lewis. "The integral membrane enzyme PagP alternates between two dynamically distinct states."  Proc. Natl. Acad. Sci. U. S. A. ., .-..

Assembly members:
PagP, polymer, 170 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
PagP: MNADEWMTTFRENIAQTWQQ PEHYDLYIPAITWHARFAYD KEKTDRYNERPWGGGFGLSR WDEKGNWHGLYAMAFKDSWN KWEPIAGYGWESTWRPLADE NFHLGLGFTAGVTARDNWNY IPLPVLLPLASVGYGPVTFQ MTYIPGTYNNGNVYFAWMRF QFLEHHHHHH

Data sets:
Data typeCount
1H chemical shifts117
13C chemical shifts232
15N chemical shifts117

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PagP in CYFOS-71

Entities:

Entity 1, PagP in CYFOS-7 170 residues - Formula weight is not available

1   METASNALAASPGLUTRPMETTHRTHRPHE
2   ARGGLUASNILEALAGLNTHRTRPGLNGLN
3   PROGLUHISTYRASPLEUTYRILEPROALA
4   ILETHRTRPHISALAARGPHEALATYRASP
5   LYSGLULYSTHRASPARGTYRASNGLUARG
6   PROTRPGLYGLYGLYPHEGLYLEUSERARG
7   TRPASPGLULYSGLYASNTRPHISGLYLEU
8   TYRALAMETALAPHELYSASPSERTRPASN
9   LYSTRPGLUPROILEALAGLYTYRGLYTRP
10   GLUSERTHRTRPARGPROLEUALAASPGLU
11   ASNPHEHISLEUGLYLEUGLYPHETHRALA
12   GLYVALTHRALAARGASPASNTRPASNTYR
13   ILEPROLEUPROVALLEULEUPROLEUALA
14   SERVALGLYTYRGLYPROVALTHRPHEGLN
15   METTHRTYRILEPROGLYTHRTYRASNASN
16   GLYASNVALTYRPHEALATRPMETARGPHE
17   GLNPHELEUGLUHISHISHISHISHISHIS

Samples:

sample_1: PagP, [U-13C; U-15N; U-2H], 1.0 mM; CYFOS-7 mM

Ex-cond_1: pH: 6.0; temperature: 318 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_1not availableEx-cond_1

Software:

No software information available

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz

Related Database Links:

BMRB 5557
PDB
DBJ BAA35265 BAB34084 BAG76214 BAI24026 BAI29493
EMBL CAP75122 CAQ31098 CAQ97476 CAR02004 CAR06827
GB AAA67555 AAB40822 AAC73723 AAG54957 AAN42294
REF NP_286349 NP_308688 NP_415155 NP_706587 NP_752642
SP D2AA19 E0J1Q4 E8Y6Y6 P37001 Q0T6N3