BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6233

Title: (poly)ubiquitin-binding region of S5a

Authors: Wang, Qinghua; Walters, Kylie

Citation: Wang, Qinghua; Walters, Kylie. "Letter to the Editor: Chemical shift assignments of the (poly)ubiquitin-binding region of the proteasome subunit S5a"  J. Biomol. NMR 30, 231-232 (2004).

Assembly members:
(poly)ubiquitin-binding region of S5a, polymer, 111 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
(poly)ubiquitin-binding region of S5a: MLGLGASDFEFGVDPSADPE LALALRVSMEEQRQRQEEEA RRAAAASAAEAGIATTGTED SDDALLKMTISQQEFGRTGL PDLSSMTEEEQIAYAMQMSL QGAEFGQAESA

Data sets:
Data typeCount
1H chemical shifts636
13C chemical shifts417
15N chemical shifts117

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1S5a1

Entities:

Entity 1, S5a 111 residues - Formula weight is not available

1   METLEUGLYLEUGLYALASERASPPHEGLU
2   PHEGLYVALASPPROSERALAASPPROGLU
3   LEUALALEUALALEUARGVALSERMETGLU
4   GLUGLNARGGLNARGGLNGLUGLUGLUALA
5   ARGARGALAALAALAALASERALAALAGLU
6   ALAGLYILEALATHRTHRGLYTHRGLUASP
7   SERASPASPALALEULEULYSMETTHRILE
8   SERGLNGLNGLUPHEGLYARGTHRGLYLEU
9   PROASPLEUSERSERMETTHRGLUGLUGLU
10   GLNILEALATYRALAMETGLNMETSERLEU
11   GLNGLYALAGLUPHEGLYGLNALAGLUSER
12   ALA

Samples:

sample1: (poly)ubiquitin-binding region of S5a, [U-13C; U-15N], 0.35 mM

Ex-cond_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCAsample1not availableEx-cond_1
HNCOsample1not availableEx-cond_1
TOCSY-15N-HSQCsample1not availableEx-cond_1
15N-HSQCsample1not availableEx-cond_1
15-NOESYsample1not availableEx-cond_1

Software:

No software information available

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAA97576 BAA97577 BAA97581 BAI45716
GB AAB54057 AAB68598 AAC50433 AAG09200 AAH02365
PRF 2207223A
REF NP_001013616 NP_001038000 NP_001098398 NP_001130032 NP_001130036
SP P55036 Q58DA0
TPG DAA31643