BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6225

Title: 1H, 15N, and 13C Resonance Assignment of the Amino-terminal Domain of Tfb1 subunit of yeast TFIIH

Authors: Nguyen, Bao; Di Lello, Paola; Legault, Pascale; Ominchinski, James

Citation: Nguyen, Bao; Di Lello, Paola; Legault, Pascale; Ominchinski, James. "Letter to the Editor: 1H, 15N, and 13C Resonance Assignment of the Amino-terminal Domain of Tfb1 subunit of yeast TFIIH"  J. Biomol. NMR 31, 173-174 (2005).

Assembly members:
Yeast Tfb1, polymer, 119 residues, Formula weight is not available

Natural source:   Common Name: Baker   Taxonomy ID: 4932   Superkingdom: not available   Kingdom: not available   Genus/species: Eukaryota Fungi

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Yeast Tfb1: PSHSGAAIFEKVSGIIAINE DVSPAELTWRSTDGDKVHTV VLSTIDKLQATPASSEKMML RLIGKVDESKKRKDNEGNEV VPKPQRHMFSFNNRTVMDNI KMTLQQIISRYKDADGNSS

Data sets:
Data typeCount
1H chemical shifts807
13C chemical shifts497
15N chemical shifts124

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Tfb11

Entities:

Entity 1, Tfb1 119 residues - Formula weight is not available

1   PROSERHISSERGLYALAALAILEPHEGLU
2   LYSVALSERGLYILEILEALAILEASNGLU
3   ASPVALSERPROALAGLULEUTHRTRPARG
4   SERTHRASPGLYASPLYSVALHISTHRVAL
5   VALLEUSERTHRILEASPLYSLEUGLNALA
6   THRPROALASERSERGLULYSMETMETLEU
7   ARGLEUILEGLYLYSVALASPGLUSERLYS
8   LYSARGLYSASPASNGLUGLYASNGLUVAL
9   VALPROLYSPROGLNARGHISMETPHESER
10   PHEASNASNARGTHRVALMETASPASNILE
11   LYSMETTHRLEUGLNGLNILEILESERARG
12   TYRLYSASPALAASPGLYASNSERSER

Samples:

sample_1: Yeast Tfb1 1.2 mM

sample_2: Yeast Tfb1, [U-98% 15N], 1.2 mM

sample_3: Yeast Tfb1, [U-98% 13C; U-98% 15N], 1.2 mM

Ex-cond_1: pH*: 6.5; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
1H-13C CT-HSQCnot availablenot availablenot available
1H-15N NOESY-HSQCnot availablenot availablenot available
HNCACBnot availablenot availablenot available
(HB)CBCA(CO)NNHnot availablenot availablenot available
HNCOnot availablenot availablenot available
H(CCO)NNH-TOCSYnot availablenot availablenot available
C(CO)NNH-TOCSYnot availablenot availablenot available
1H-13C HMQC-NOESYnot availablenot availablenot available
1H-15N NOESY_HSQCnot availablenot availablenot available
NOESYnot availablenot availablenot available
DQF-COSYnot availablenot availablenot available
(HB)CB(CGCD)HDnot availablenot availablenot available
(HB)CB(CGCDCE)HEnot availablenot availablenot available

Software:

NMRPIPE - data processing

NMRVIEW v5.3 - data analysis

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Varian INOVA 600 MHz
  • Varian INOVA 500 MHz

Related Database Links:

BMRB 18229 18842 19791
PDB
DBJ GAA22531
EMBL CAY78811
GB AAA35143 AAB64747 AAU09707 AHY75284 EDN60639
REF NP_010597
SP P32776
TPG DAA12150