BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6216

Title: Solution structure of the third zinc finger domain of FOG-1   PubMed: 15234987

Authors: Simpson, R.; Lee, S.; Bartle, N.; Matthews, J.; Mackay, J.; Crossley, M.

Citation: Simpson, R.; Lee, S.; Bartle, N.; Sum, E.; Visvader, J.; Matthews, J.; Mackay, J.; Crossley, M.. "A classic zinc finger from friend of GATA mediates an interaction with the coiled-coil of transforming acidic coiled-coil 3"  J. Biol. Chem. 279, 39789-39797 (2004).

Assembly members:
ZFPM1, polymer, 35 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
ZFPM1: GSSGKRPFVCRICLSAFTTK ANCARHLKVHTDTLS

Data sets:
Data typeCount
1H chemical shifts220
coupling constants31

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Zinc finger protein ZFPM11
2ZINC (II) ION2

Entities:

Entity 1, Zinc finger protein ZFPM1 35 residues - Formula weight is not available

1   GLYSERSERGLYLYSARGPROPHEVALCYS
2   ARGILECYSLEUSERALAPHETHRTHRLYS
3   ALAASNCYSALAARGHISLEULYSVALHIS
4   THRASPTHRLEUSER

Entity 2, ZINC (II) ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: ZFPM1 0.27 mM; ZnSO4 1 mM; TCEP 2 mM; H2O 95%; D2O 5%

sample_2: ZFPM1, [U-15N], 0.245 mM; ZnSO4 1 mM; TCEP 2 mM; H2O 95%; D2O 5%

sample_cond_1: ionic strength: 0 mM; pH: 5.6; pressure: 1 atm; temperature: 275 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablesample_cond_1
2D TOCSYnot availablenot availablesample_cond_1
DQF-COSYnot availablenot availablesample_cond_1
HNHAnot availablenot availablesample_cond_1

Software:

xwinnmr v2.6 - processing

XEASY v1.3.13 - data analysis

DYANA v1.5 - refinement

ARIA v1.2 - structure solution

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

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