BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6209

Title: Solution Structure of a Calmodulin-Like Calcium-Binding Domain from Arabidopsis thaliana

Authors: Song, Jikui; Zhao, Qin; Thao, Sandy; Frederick, Ronnie; Markley, John

Citation: Song, Jikui; Zhao, Qin; Thao, Sandy; Frederick, Ronnie; Markley, John. "Letter to the Editor: Solution Structure of a Calmodulin-Like Calcium-Binding Domain from Arabidopsis thaliana"  J. Biomol. NMR 30, 451-456 (2004).

Assembly members:
N-terminal domain of At3g03410.1, polymer, 67 residues, Formula weight is not available

Natural source:   Common Name: thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
N-terminal domain of At3g03410.1: SSAKRVFEKFDKNKDGKLSL DEFREVALAFSPYFTQEDIV KFFEEIDVDGNGELNADEFT SCIEKML

Data sets:
Data typeCount
1H chemical shifts395
13C chemical shifts261
15N chemical shifts69
residual dipolar couplings55

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Calmodulin like protein1

Entities:

Entity 1, Calmodulin like protein 67 residues - Formula weight is not available

1   SERSERALALYSARGVALPHEGLULYSPHE
2   ASPLYSASNLYSASPGLYLYSLEUSERLEU
3   ASPGLUPHEARGGLUVALALALEUALAPHE
4   SERPROTYRPHETHRGLNGLUASPILEVAL
5   LYSPHEPHEGLUGLUILEASPVALASPGLY
6   ASNGLYGLULEUASNALAASPGLUPHETHR
7   SERCYSILEGLULYSMETLEU

Samples:

sample_1: N-terminal domain of At3g03410.1, [U-15N], 1 mM; DTT 5 mM; Bis-Tris 20 mM; CaCl2 5 mM; DSS 1 mM

2: N-terminal domain of At3g03410.1, [U-15N; U-13C], 1 mM; DTT 5 mM; Bis-Tris 20 mM; CaCl2 5 mM; DSS 1 mM

3: N-terminal domain of At3g03410.1, [U-15N; U-13C], 1 mM; DTT 5 mM; Bis-Tris 20 mM; CaCl2 5 mM; DSS 1 mM; PEG 5%

Conditions_1: pH: 6.4; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCACBnot availablenot availableConditions_1
HN(CO)CACBnot availablenot availableConditions_1
N15 edited NOESYnot availablenot availableConditions_1
N15 edited TOCSYnot availablenot availableConditions_1
IPAP-HSQCnot availablenot availableConditions_1

Software:

XWINNMR v3.2, Bruker - Data collection

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

SP Q9SRP5
REF NP_186991
GB ABK32174 AAF01604
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