BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6173

Title: PfR48 final project

Authors: Snyder, David

Citation: Snyder, David; Aramini, J.; Huang, Y.; Xiao, R.; Cort, J.; Shastry, R.; Ma, L.; Liu, J.; Rost, B.; Acton, T.; Kennedy, M.; Montelione, G.. "Solution Structure of the 50S Ribosomal Protein L35Ae from Pyrococcus furiosus: Northeast Strucutral Genomics Consortium target: Pfr48"  . ., .-..

Assembly members:
PfR48, polymer, 95 residues, 9735.5 Da.

Natural source:   Common Name: Pyrococcus furiosus   Taxonomy ID: 2261   Superkingdom: Archaea   Kingdom: not available   Genus/species: Pyrococcus furiosus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
PfR48: MRIKGVVLSYRRSKENQHNN VMIIKPLDVNSREEASKLIG RLVLWKSPSGKILKGKIVRV HGTKGAVRARFEKGLPGQAL GDYVEIVLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts396
15N chemical shifts93
1H chemical shifts656
coupling constants42

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PfR481

Entities:

Entity 1, PfR48 95 residues - 9735.5 Da.

1   METARGILELYSGLYVALVALLEUSERTYR
2   ARGARGSERLYSGLUASNGLNHISASNASN
3   VALMETILEILELYSPROLEUASPVALASN
4   SERARGGLUGLUALASERLYSLEUILEGLY
5   ARGLEUVALLEUTRPLYSSERPROSERGLY
6   LYSILELEULYSGLYLYSILEVALARGVAL
7   HISGLYTHRLYSGLYALAVALARGALAARG
8   PHEGLULYSGLYLEUPROGLYGLNALALEU
9   GLYASPTYRVALGLUILEVALLEUGLUHIS
10   HISHISHISHISHIS

Samples:

sample_PfR48.NC.RX.7.85: PfR48, [U-15N], 1.07 mM; NaCl 100 mM; CaCl2 5 mM

sample_PfR48.NC5.LYD.III.3-6.5: PfR48, [U-15N], 0.29 mM; NaCl 100 mM; CaCl2 5 mM

sample_PfR48.PfR48-21.1-N-GF-6.5: PfR48, [U-15N], 0.5 mM; NaCl 100 mM; CaCl2 5 mM

Sample_condition_list_1: ionic strength: 0.1 M; pH: 6.5; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
HNCAsample_PfR48.NC.RX.7.85not availableSample_condition_list_1
HNCACBsample_PfR48.NC.RX.7.85not availableSample_condition_list_1
HNCOsample_PfR48.NC.RX.7.85not availableSample_condition_list_1
NH_HSQCsample_PfR48.NC.RX.7.85not availableSample_condition_list_1
NH_HSQCsample_PfR48.NC.RX.7.85not availableSample_condition_list_1
HNcoCAsample_PfR48.NC.RX.7.85not availableSample_condition_list_1
HNcoCACBsample_PfR48.NC.RX.7.85not availableSample_condition_list_1
HAcacoNHsample_PfR48.NC.RX.7.85not availableSample_condition_list_1
HAcaNHsample_PfR48.NC.RX.7.85not availableSample_condition_list_1
HNCACB (tau c)sample_PfR48.NC.RX.7.85not availableSample_condition_list_1
HNHAsample_PfR48.NC.RX.7.85not availableSample_condition_list_1
CCONHsample_PfR48.NC.RX.7.85not availableSample_condition_list_1
HCCH-COSYsample_PfR48.NC.RX.7.85not availableSample_condition_list_1
HCCH-TOCSYsample_PfR48.NC.RX.7.85not availableSample_condition_list_1
hetNOE (t0)sample_PfR48.NC.RX.7.85not availableSample_condition_list_1
hetNOE (t3)sample_PfR48.NC.RX.7.85not availableSample_condition_list_1
CH_HSQC (aliph)sample_PfR48.NC.RX.7.85not availableSample_condition_list_1
CH_HSQC (aliph)sample_PfR48.NC.RX.7.85not availableSample_condition_list_1
hbCBcgcdceHEsample_PfR48.NC.RX.7.85not availableSample_condition_list_1
HBCBcgcdHDsample_PfR48.NC.RX.7.85not availableSample_condition_list_1
MUSIC_gly_CAsample_PfR48.NC.RX.7.85not availableSample_condition_list_1
MUSIC_gly_COsample_PfR48.NC.RX.7.85not availableSample_condition_list_1
CH_HSQC (arom)sample_PfR48.NC.RX.7.85not availableSample_condition_list_1
15N-edited_NOESYsample_PfR48.NC.RX.7.85not availableSample_condition_list_1
13C_edited_NOESY (aliph)sample_PfR48.NC.RX.7.85not availableSample_condition_list_1
13C_edited_NOESY (D2O aliph)sample_PfR48.NC.RX.7.85not availableSample_condition_list_1
13C_edited_NOESY (arom)sample_PfR48.NC.RX.7.85not availableSample_condition_list_1
HCCcoNH-TOCSYsample_PfR48.NC.RX.7.85not availableSample_condition_list_1
4D-C13-NOESYsample_PfR48.NC.RX.7.85not availableSample_condition_list_1
NH_HSQC (larger SW1)sample_PfR48.NC5.LYD.III.3-6.5not availableSample_condition_list_1
CH_HSQCsample_PfR48.NC5.LYD.III.3-6.5not availableSample_condition_list_1
NH_HSQC (15 min)sample_PfR48.PfR48-21.1-N-GF-6.5not availableSample_condition_list_1

Software:

VNMR_6.1C - data collection

NMRPipe - data processing

SPARKY - spectral visualization

AutoAssign -

AutoStruct - automated structural determination

SPINS - integrated spectral analysis software

NMR spectrometers:

  • varian Baker 600 MHz
  • varian UNITY Inova 500 MHz
  • Varian INOVA 750 MHz
  • varian INOVA 800 MHz
  • varian UNITY Inova 600 MHz

Related Database Links:

PDB
GB AAL81996 AFN04768
REF NP_579601 WP_011013011 YP_006493060
SP P20299 Q8TZV6