BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6172

Title: Solution structure of a cyanobacterial copper metallochaperone, ScAtx1   PubMed: 15075318

Authors: Banci, L.; Bertini, I.; Ciofi-Baffoni, S.; Su, X.; Borrelly, G.; Robinson, N.

Citation: Banci, L.; Bertini, I.; Ciofi-Baffoni, S.; Su, X.; Borrelly, G.; Robinson, N.. "Solution structures of a cyanobacterial copper metallochaperone: insight into an atypical copper binding motif"  J. Biol. Chem. ., .-. (2004).

Assembly members:
apoScAtx1, polymer, 64 residues, Formula weight is not available

Natural source:   Common Name: Synechocystis sp. PCC 6803   Taxonomy ID: 1148   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Synechocystis Synechocystis sp. PCC 6803

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
apoScAtx1: MTIQLTVPTIACEACAEAVT KAVQNEDAQATVQVDLTSKK VTITSALGEEQLRTAIASAG HEVE

Data sets:
Data typeCount
15N chemical shifts67
1H chemical shifts410

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1apo ScAtx11

Entities:

Entity 1, apo ScAtx1 64 residues - Formula weight is not available

1   METTHRILEGLNLEUTHRVALPROTHRILE
2   ALACYSGLUALACYSALAGLUALAVALTHR
3   LYSALAVALGLNASNGLUASPALAGLNALA
4   THRVALGLNVALASPLEUTHRSERLYSLYS
5   VALTHRILETHRSERALALEUGLYGLUGLU
6   GLNLEUARGTHRALAILEALASERALAGLY
7   HISGLUVALGLU

Samples:

sample_1: apoScAtx1, [U-15N], 0.7 mM; phosphate 50 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 50 mM; pH: 7.0 na; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1not availablesample_cond_1
3D 15N-separated NOESYsample_1not availablesample_cond_1
2D TOCSYsample_1not availablesample_cond_1
HNHAsample_1not availablesample_cond_1

Software:

xwinnmr v2.6 - processing

XEASY v1.3 - structure solution

DYANA v1.5 - structure solution

AMBER v5.0 - refinement

NMR spectrometers:

  • Bruker AVANCE 500 MHz

Related Database Links:

PDB
DBJ BAA17240 BAK49413 BAL28412 BAL31581 BAL34750
GB AGF50929
REF NP_440560 WP_010871869 YP_005382427 YP_005385596 YP_005408303