BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6139

Title: Solution Structure of Ole e 6, an allergen from olive tree pollen   PubMed: 15247256

Authors: Trevino, Miguel; Garcia-Mayoral, M Flor; Barral, Patricia; Villalba, Mayte; Rico, Manuel; Santoro, Jorge; Rodriguez, Rosalia; Bruix, Marta

Citation: Trevino, Miguel; Garcia-Mayoral, M. Flor; Barral, Patricia; Villalba, Mayte; Santoro, Jorge; Rico, Manuel; Rodriguez, Rosalia; Bruix, Marta. "NMR Solution Structure of Ole e 6, a major allergen from olive tree pollen"  J. Biol. Chem. ., .-. (2004).

Assembly members:
Olea europea pollen allergen ole e 6, polymer, 50 residues, 5810 Da.

Natural source:   Common Name: olive tree   Taxonomy ID: 4146   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Olea europea

Experimental source:   Production method: recombinant technology   Host organism: Pichia pastoris

Entity Sequences (FASTA):
Olea europea pollen allergen ole e 6: DEAQFKECYDTCHKECSDKG NGFTFCEMKCDTDCSVKDVK EKLENYKPKN

Data sets:
Data typeCount
1H chemical shifts614
15N chemical shifts52

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1ole e 61

Entities:

Entity 1, ole e 6 50 residues - 5810 Da.

1   ASPGLUALAGLNPHELYSGLUCYSTYRASP
2   THRCYSHISLYSGLUCYSSERASPLYSGLY
3   ASNGLYPHETHRPHECYSGLUMETLYSCYS
4   ASPTHRASPCYSSERVALLYSASPVALLYS
5   GLULYSLEUGLUASNTYRLYSPROLYSASN

Samples:

sample_1: Olea europea pollen allergen ole e 60.6 – 1.0 mM; H2O 95%; D2O 5%

sample_2: Olea europea pollen allergen ole e 60.6 – 1.0 mM; D2O 100%

sample_3: Olea europea pollen allergen ole e 6, [U-15N], 0.6 – 1.0 mM; H2O 95%; D2O 5%

cond_pH6_H2O: pH: 6.0; temperature: 308 K

cond_pH6_D2O: pH: 6.0; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
1H-COSYnot availablenot availablenot available
1H-TOCSYnot availablenot availablenot available
1H-NOESYnot availablenot availablenot available
1H-15N-HSQCnot availablenot availablenot available
1H-15N-NOESY-HSQCnot availablenot availablenot available

Software:

XWINNMR - raw spectral data proccesing

ANSIG v3.3 - peak picking, peak assigments, crosspeak area calculations

CALIBA - upper limits calculations

DYANA v1.5 - structures calculations

GLOMSA - stereospecific assigments

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
GB AAB66909 AAO33896 AAO34670
SP O24172