BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5978

Title: Structure and function of the membrane domain of hepatitis C virus nonstructural protein 5A   PubMed: 15247283

Authors: Ramboarina, Stephanie; Brass, Volker; Krieger, Nicole; Montserret, Roland; Ficheux, Damien; Blum, Hubert; Bartenschlager, Ralf; Penin, Francois; Moradpour, Darius

Citation: Penin, Francois; Brass, Volker; Appel, Nicole; Ramboarina, Stephanie; Montserret, Roland; Ficheux, Damien; Blum, Hubert; Bartenschlager, Ralf; Moradpour, Darius. "Structure and function of the membrane anchor domain of hepatitis C virus nonstructural protein 5A "  J. Biol. Chem. 279, 40835-40843 (2004).

Assembly members:
HCV NS5A N-terminal, polymer, 31 residues, 3764 Da.

Natural source:   Common Name: Hepatitis C virus   Taxonomy ID: 11103   Superkingdom: Viruses   Kingdom: not available   Genus/species: Hepacivirus Hepatitis C virus

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
HCV NS5A N-terminal: SGSWLRDIWDWICEVLSDFK TWLKAKLMPQL

Data sets:
Data typeCount
1H chemical shifts718
13C chemical shifts221

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1NS5A, 1-311

Entities:

Entity 1, NS5A, 1-31 31 residues - 3764 Da.

1   SERGLYSERTRPLEUARGASPILETRPASP
2   TRPILECYSGLUVALLEUSERASPPHELYS
3   THRTRPLEULYSALALYSLEUMETPROGLN
4   LEU

Samples:

Sample_tfe: HCV NS5A N-terminal 1.2 mM; DTT, [U-2H], 10 mM; trifluoroethanol-d2 (CF3CD2OH) 50 % v/v; H2O 50 % v/v

Sample_sds: HCV NS5A N-terminal 1.2 mM; DTT, [U-2H], 10 mM; sodium dodecyl sulfate, [U-2H], 100 mM

Sample_dpc: HCV NS5A N-terminal 1.2 mM; DTT, [U-2H], 10 mM; dodecylphosphocholine, [U-2H], 100 mM

condition_tfe: pH: 4.5; temperature: 293 K

condition_sds: pH: 6.0; temperature: 313 K

condition_dpc: pH: 6.0; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
DQF-COSYnot availablenot availablenot available
NOESYnot availablenot availablenot available
TOCSYnot availablenot availablenot available
1H-13C HSQCnot availablenot availablenot available

Software:

VNMR v6.1 - processing, assigments

NMR spectrometers:

  • Varian UnityPlus 500 MHz

Related Database Links:

PDB
DBJ BAI23205
EMBL CAC03609 CAL46810 CAM82857 CAM82858 CAM82860
GB AAA45534 AAA45676 AAA45677 AAA86588 AAA86589
REF NP_671491 NP_751927
SP P26664 P27958