BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5842

Title: Backbone and side chain 1H, 13C, and 15N chemical shift assignments for Haemophilus influenza protein IR24   PubMed: 15522305

Authors: Ramelot, Theresa; Kennedy, Michael

Citation: Ramelot, T.; Cort, J.; Goldsmith-Fischman, S.; Kornhaber, G.; Xiao, R.; Shastry, R.; Acton, T.; Honig, B.; Montelione, G.; Kennedy, M.. "Solution NMR structure of the iron-sulfur cluster assembly protein U (IscU) with zinc bound at the active site"  J. Mol. Biol. 344, 567-583 (2004).

Assembly members:
IR24, polymer, 134 residues, 14500 Da.

Natural source:   Common Name: haemophilus influenza   Taxonomy ID: 727   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Haemophilus influenza

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
IR24: MAYSEKVIDHYENPRNVGSL DKKDSNVGTGMVGAPACGDV MQLQIKVDDNGIIEDAKFKT YGCGSAIASSSLITEWVKGK SLEEAGAIKNSQIAEELELP PVKVHCSILAEDAIKAAIAD YKAKQGLEHHHHHH

Data sets:
Data typeCount
1H chemical shifts723
13C chemical shifts448
15N chemical shifts116

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1IR241

Entities:

Entity 1, IR24 134 residues - 14500 Da.

1   METALATYRSERGLULYSVALILEASPHIS
2   TYRGLUASNPROARGASNVALGLYSERLEU
3   ASPLYSLYSASPSERASNVALGLYTHRGLY
4   METVALGLYALAPROALACYSGLYASPVAL
5   METGLNLEUGLNILELYSVALASPASPASN
6   GLYILEILEGLUASPALALYSPHELYSTHR
7   TYRGLYCYSGLYSERALAILEALASERSER
8   SERLEUILETHRGLUTRPVALLYSGLYLYS
9   SERLEUGLUGLUALAGLYALAILELYSASN
10   SERGLNILEALAGLUGLULEUGLULEUPRO
11   PROVALLYSVALHISCYSSERILELEUALA
12   GLUASPALAILELYSALAALAILEALAASP
13   TYRLYSALALYSGLNGLYLEUGLUHISHIS
14   HISHISHISHIS

Samples:

sample_1: IR24, [U-15N; U-13C], 1 mM; MES 20 mM; NaCl 100 mM

sample_2: IR24, [U-15N], 1 mM; MES 20 mM; NaCl 100 mM

sample_3: IR24, [U-5% 13C; U-100% 15N], 1 mM; MES 20 mM; NaCl 100 mM

cond_1: pH: 6.5; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablecond_1
1H-13C HSQC (aliph)not availablenot availablecond_1
15N-edited NOESY-HQSCnot availablenot availablecond_1
HCC-TOCSY-NNHnot availablenot availablecond_1
HCCH-TOCSYnot availablenot availablecond_1
13C-edited-NOESY-HSQC (aliph)not availablenot availablecond_1
CBCACOCAHAnot availablenot availablecond_1
HNCAnot availablenot availablecond_1
HN(CO)CAnot availablenot availablecond_1
HNCOnot availablenot availablecond_1
HNCACBnot availablenot availablecond_1
CBCA(CO)NHnot availablenot availablecond_1
CC-TOCSY-NNHnot availablenot availablecond_1
HNHAnot availablenot availablecond_1
4D CC-NOESYnot availablenot availablecond_1
1H-13C HSQC (arom)not availablenot availablecond_1
13C-edited-NOESY-HSQC (arom)not availablenot availablecond_1
1H-13C HSQC (alpha)not availablenot availablecond_1

Software:

Felix v98 - processing data

XPLOR NIH v2.0.4 - structure calculations

NMR spectrometers:

  • INOVA Varian 500 MHz
  • INOVA Varian 600 MHz
  • INOVA Varian 750 MHz
  • INOVA Varian 800 MHz

Related Database Links:

PDB
EMBL CBW28676 CBY80560 CBY86563
GB AAC22034 AAX87432 ABQ97710 ABQ99885 ADO80188
PIR C64064
REF NP_438538 WP_005649165 WP_005654861 WP_005666597 WP_005691745
SP Q57074