BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5796

Title: Solution Structure of a Putative Ribosome Binding-Protein from Mycoplasma pneumoniae and Comparison to a Distant Homolog   PubMed: 15185964

Authors: Rubin, Seth; Pelton, Jeffery; Yokota, Hisao; Kim, Rosalind; Wemmer, David

Citation: Rubin, Seth; Pelton, Jeffery; Yokota, Hisao; Kim, Rosalind; Wemmer, David. "Solution Structure of a Putative Ribosome Binding Protein from Mycoplasma pneumoniae and Comparison to a Distant Homolog "  J. Struct. Funct. Genomics 4, 235-243 (2003).

Assembly members:
MPN156, polymer, 117 residues, Formula weight is not available

Natural source:   Common Name: Mycoplasma pneumoniae   Taxonomy ID: 2104   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Mycoplasma pneumoniae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
MPN156: GMASYKKERLENDIIRLINR TVIHEIYNETVKTGHVTHVK LSDDLLHVTVYLDCYNREQI DRVVGAFNQAKGVFSRVLAH NLYLAKAVQIHFVKDKAIDN AMRIESIINSLKKSKPN

Data sets:
Data typeCount
1H chemical shifts586
13C chemical shifts307
15N chemical shifts96
coupling constants65

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MPN1561

Entities:

Entity 1, MPN156 117 residues - Formula weight is not available

1   GLYMETALASERTYRLYSLYSGLUARGLEU
2   GLUASNASPILEILEARGLEUILEASNARG
3   THRVALILEHISGLUILETYRASNGLUTHR
4   VALLYSTHRGLYHISVALTHRHISVALLYS
5   LEUSERASPASPLEULEUHISVALTHRVAL
6   TYRLEUASPCYSTYRASNARGGLUGLNILE
7   ASPARGVALVALGLYALAPHEASNGLNALA
8   LYSGLYVALPHESERARGVALLEUALAHIS
9   ASNLEUTYRLEUALALYSALAVALGLNILE
10   HISPHEVALLYSASPLYSALAILEASPASN
11   ALAMETARGILEGLUSERILEILEASNSER
12   LEULYSLYSSERLYSPROASN

Samples:

sample_15N: MPN156, [U-100% 15N], 1 mM; sodium phosphate 25 mM; sodium chloride 50 mM; DTT 1 mM; EDTA 1 mM

sample_15N_13C: MPN156, [U-100% 15N; U-100% 13C], 1 mM; sodium phosphate 25 mM; sodium chloride 50 mM; DTT 1 mM; EDTA 1 mM

sample_13C: MPN156, [U-10% 13C], 1 mM; sodium phosphate 25 mM; sodium chloride 50 mM; DTT 1 mM; EDTA 1 mM

Sample_conditions: pH: 6.6 na; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HBHA(CO)NHnot availablenot availablenot available
HNHAnot availablenot availablenot available
15-N NOESYnot availablenot availablenot available
HCCH TOCSYnot availablenot availablenot available

Software:

NMRPIPE v1.0 - Processing

NMRVIEW v5.0.4 - Analysis

NMR spectrometers:

  • BRUKER DRX 500 MHz
  • BRUKER DRX 600 MHz

Related Database Links:

PDB
DBJ BAL21730
GB AAB96323 ADK86785 AGC04083
REF NP_109844 WP_010874513 YP_005175394 YP_005881189 YP_007347770
SP P75589