BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5770

Title: Structural basis for simultaneous binding of two carboxy-terminal peptides of plant glutamate decarboxylase to calmodulin   PubMed: 12684008

Authors: Yap, Kyoko; Yuan, Tao; Mal, Tapas; Vogel, Hans; Ikura, Mitsu

Citation: Yap, Kyoko; Yuan, Tao; Mal, Tapas; Vogel, Hans; Ikura, Mitsu. "Structural basis for simultaneous binding of two carboxy-terminal peptides of plant glutamate decarboxylase to calmodulin "  J. Mol. Biol. 328, 193-204 (2003).

Assembly members:
Calmodulin, polymer, 148 residues, Formula weight is not available
Glutamate decarboxylase, polymer, 28 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:   Common Name: African clawed frog   Taxonomy ID: 8355   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Xenopus laevis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Calmodulin: ADQLTEEQIAEFKEAFSLFD KDGDGTITTKELGTVMRSLG QNPTEAELQDMINEVDADGN GTIDFPEFLTMMARKMKDTD SEEEIREAFRVFDKDGNGYI SAAELRHVMTNLGEKLTDEE VDEMIREADIDGDGQVNYEE FVQMMTAK
Glutamate decarboxylase: GSHKKTDSEVQLEMITAWKK FVEEKKKK

Data sets:
Data typeCount
1H chemical shifts1437
13C chemical shifts908
15N chemical shifts210

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1calmodulin1
2glutamate decarboxylase peptide A2
3glutamate decarboxylase peptide B2
4calcium ion I3
5calcium ion II3
6calcium ion III3
7calcium ion IV3

Entities:

Entity 1, calmodulin 148 residues - Formula weight is not available

1   ALAASPGLNLEUTHRGLUGLUGLNILEALA
2   GLUPHELYSGLUALAPHESERLEUPHEASP
3   LYSASPGLYASPGLYTHRILETHRTHRLYS
4   GLULEUGLYTHRVALMETARGSERLEUGLY
5   GLNASNPROTHRGLUALAGLULEUGLNASP
6   METILEASNGLUVALASPALAASPGLYASN
7   GLYTHRILEASPPHEPROGLUPHELEUTHR
8   METMETALAARGLYSMETLYSASPTHRASP
9   SERGLUGLUGLUILEARGGLUALAPHEARG
10   VALPHEASPLYSASPGLYASNGLYTYRILE
11   SERALAALAGLULEUARGHISVALMETTHR
12   ASNLEUGLYGLULYSLEUTHRASPGLUGLU
13   VALASPGLUMETILEARGGLUALAASPILE
14   ASPGLYASPGLYGLNVALASNTYRGLUGLU
15   PHEVALGLNMETMETTHRALALYS

Entity 2, glutamate decarboxylase peptide A 28 residues - Formula weight is not available

1   GLYSERHISLYSLYSTHRASPSERGLUVAL
2   GLNLEUGLUMETILETHRALATRPLYSLYS
3   PHEVALGLUGLULYSLYSLYSLYS

Entity 3, calcium ion I - Ca - 40.078 Da.

1   CA

Samples:

sample_1: Calmodulin, [U-15N], 1.0 mM; Glutamate decarboxylase, [U-50% 13C], 2.2 mM; KCl 100 mM; CaCl2 10 mM; D2O 9%; H2O 91%

sample_2: Calmodulin, [U-15N; U-13C], 1.0 mM; Glutamate decarboxylase, [U-50% 13C], 2.2 mM; KCl 100 mM; CaCl2 10 mM; D2O 9%; H2O 91%

Ex-cond_1: pH: 6.3; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
1H, 15N HSQCnot availablenot availableEx-cond_1
1H, 13C HSQCnot availablenot availableEx-cond_1
HNCACBnot availablenot availableEx-cond_1
CBCA(CO)NHnot availablenot availableEx-cond_1
HNCOnot availablenot availableEx-cond_1
HCCH-TOCSYnot availablenot availableEx-cond_1
HCC-TOCSY-NNHnot availablenot availableEx-cond_1
(HB)CB(CGCD)HDnot availablenot availableEx-cond_1
(HB)CB(CGCE)HEnot availablenot availableEx-cond_1
3D simultaneous 13C,15N-edited NOESY-HSQCnot availablenot availableEx-cond_1
13C-edited, 13C-filtered NOESY-HSQCnot availablenot availableEx-cond_1

Software:

No software information available

NMR spectrometers:

  • Varian Unity-Plus 500 MHz
  • Varian Unity-Plus 600 MHz

Related Database Links:

PDB
GB AAA33709 AAA33710
SP Q07346
GenBank AAA33710 AAA33709
SWISS-PROT Q07346