BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5767

Title: 1H NMR structure determination of Viscotoxin C1   PubMed: 14580196

Authors: Molinari, H.; Romagnoli, S.; Fogolari, F.; Catalano, M.; Urech, K.; Giannattasio, M.; Ragona, L.

Citation: Romagnoli, S.; Fogolari, F.; Catalano, M.; Zetta, L.; Schaller, G.; Urech, K.; Giannattasio, M.; Ragona, L.; Molinari, H.. "NMR solution structure of viscotoxin C1 from Viscum album species Coloratum ohwi: toward a structure-function analysis of viscotoxins"  Biochemistry 42, 12503-12510 (2003).

Assembly members:
Cue2, polymer, 46 residues, Formula weight is not available

Natural source:   Common Name: European mistletoe   Taxonomy ID: 3972   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Viscum album

Experimental source:   Production method: prufified from the natural source

Entity Sequences (FASTA):
Cue2: KSCCPNTTGRNIYNTCRFAG GSRERCAKLSGCKIISASTC PSDYPK

Data sets:
Data typeCount
1H chemical shifts278

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1protein Cue21

Entities:

Entity 1, protein Cue2 46 residues - Formula weight is not available

1   LYSSERCYSCYSPROASNTHRTHRGLYARG
2   ASNILETYRASNTHRCYSARGPHEALAGLY
3   GLYSERARGGLUARGCYSALALYSLEUSER
4   GLYCYSLYSILEILESERALASERTHRCYS
5   PROSERASPTYRPROLYS

Samples:

sample_1: Cue2 0.7 mM; phosphate buffer 50 mM; H20 90%; D2O 10%

sample_2: Cue2 0.7 mM; phosphate buffer 50 mM; D2O 100%

sample_cond_1: ionic strength: 50 mM; pH: 3.6; pressure: 1 atm; temperature: 285 K

sample_cond_2: ionic strength: 50 mM; pH: 3.6; pressure: 1 atm; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
2D TOCSYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
DQF-COSYnot availablenot availablenot available

Software:

DYANA v1.4 - structure solution

DISCOVER v1997 version - refinement, structure solution

xwinnmr v2.6 - processing

XEASY v1.3 - data analysis

NMR spectrometers:

  • Bruker DMX 500 MHz

Related Database Links:

PDB
SP P83554