BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5766

Title: Solution Structure of the Lyase Domain of Human DNA Polymerase Lambda   PubMed: 12911298

Authors: DeRose, E.; Kirby, T.; Mueller, G.; Bebenek, K.; Garcia-Diaz, M.; Blanco, L.; Kunkel, T.; London, R.

Citation: DeRose, E.; Kirby, T.; Mueller, G.; Bebenek, K.; Garcia-Diaz, M.; Blanco, L.; Kunkel, T.; London, R.. "Solution Structure of the Lyase Domain of Human DNA Polymerase Lambda"  Biochemistry 42, 9564-9574 (2003).

Assembly members:
50S Ribosomal Protein L18, polymer, 87 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
50S Ribosomal Protein L18: MAQPSSQKATNHNLHITEKL EVLAKAYSVQGDKWRALGYA KAINALKSFHKPVTSYQEAC SIPGIGKRMAEKIIEILESG HLRKLDH

Data sets:
Data typeCount
1H chemical shifts440
13C chemical shifts328
15N chemical shifts76

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
150S Ribosomal Protein L181

Entities:

Entity 1, 50S Ribosomal Protein L18 87 residues - Formula weight is not available

1   METALAGLNPROSERSERGLNLYSALATHR
2   ASNHISASNLEUHISILETHRGLULYSLEU
3   GLUVALLEUALALYSALATYRSERVALGLN
4   GLYASPLYSTRPARGALALEUGLYTYRALA
5   LYSALAILEASNALALEULYSSERPHEHIS
6   LYSPROVALTHRSERTYRGLNGLUALACYS
7   SERILEPROGLYILEGLYLYSARGMETALA
8   GLULYSILEILEGLUILELEUGLUSERGLY
9   HISLEUARGLYSLEUASPHIS

Samples:

sample_1: 50S Ribosomal Protein L18, [U-15N; U-13C], 2 mM; Tris-d11 5 mM; NaCl 100 mM; DTT 1 mM; NaN3 5 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 7; temperature: 298 K; ionic strength: 110 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available

Software:

VNMR v6.1C - collection

NMRPipe v2.1 - processing

NMRView v5.0.4 - data analysis

CNS v1.0 - structure solution

ARIA v1.1 - structure solution, refinement

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAB13852 BAB14050 BAE02437 BAG10573 BAK63678
EMBL CAB65074
GB AAF27541 AAG22519 AAH68529 AAM77696 ADQ01128
REF NP_001167555 NP_001253835 NP_001267179 NP_001270218 NP_037406
SP Q4R380 Q9UGP5