BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5762

Title: The alternatively spliced PDZ2 domain of PTP-BL, PDZ2as   PubMed: 14725761

Authors: Walma, Tine; Aelen, Jan; Oostendorp, M.; Vuister, Geerten

Citation: Walma, Tine; Aelen, Jan; Nabuurs, S.; Oostendorp, Marlies; van den Berk, L.; Hendriks, W.; Vuister, Geerten. "A Closed Binding Pocket and Global Destabilization Modify the Binding Properties of an Alternatively Spliced Form of the Second PDZ Domain of PTP-BL"  Structure (Cambridge, MA, U. S.) 12, 11-20 (2004).

Assembly members:
Second PDZ Domain from PTP-BL, polymer, 99 residues, 10355 Da.

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Second PDZ Domain from PTP-BL: KPGDTFEVELAKTDGSLGIS VTVLFDKGGVNTSVRHGGIY VKAIIPKGAAESDGRIHKGD RVLAVNGVSLEGATHKQAVE TLRNTGQVVHLLLEKGQVP

Data sets:
Data typeCount
13C chemical shifts297
15N chemical shifts108
1H chemical shifts671
coupling constants58
heteronuclear NOE values90
H exchange protection factors55
residual dipolar couplings66
T1 relaxation values88
T2 relaxation values86

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1PDZ2-AS1

Entities:

Entity 1, PDZ2-AS 99 residues - 10355 Da.

1   LYSPROGLYASPTHRPHEGLUVALGLULEU
2   ALALYSTHRASPGLYSERLEUGLYILESER
3   VALTHRVALLEUPHEASPLYSGLYGLYVAL
4   ASNTHRSERVALARGHISGLYGLYILETYR
5   VALLYSALAILEILEPROLYSGLYALAALA
6   GLUSERASPGLYARGILEHISLYSGLYASP
7   ARGVALLEUALAVALASNGLYVALSERLEU
8   GLUGLYALATHRHISLYSGLNALAVALGLU
9   THRLEUARGASNTHRGLYGLNVALVALHIS
10   LEULEULEUGLULYSGLYGLNVALPRO

Samples:

sample_1: Second PDZ Domain from PTP-BL, [U-2H; U-15N; U-13C], 1.4 mM; K2HPO4/KH2PO4 50 mM; KCl 50 mM

sample_2: Second PDZ Domain from PTP-BL, [U-2H; U-15N], 1.1 mM; CH3COO- 50 mM; KCl 50 mM

condition_1: ionic strength: 0.1 M; pH: 6.8; pressure: 1 atm; temperature: 298 K

condition_2: ionic strength: 0.1 M; pH: 3.5; pressure: 1 atm; temperature: 281.5 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Varian Inova 800 MHz

Related Database Links:

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