BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5757

Title: 13C, 15N solid state NMR chemical shift assignments for the microcrystallin Crh domain swapped dimer

Authors: Bockmann, Anja; Lange, Adam; Galinier, Anne; Luca, Sorin; Giraud, Nicolas; Juy, Michel; Heise, Henrike; Montserret, Roland; Penin, Francois; Baldus, Marc

Citation: Bockmann, Anja; Lange, Adam; Galinier, Anne; Luca, Sorin; Giraud, Nicolas; Juy, Michel; Heise, Henrike; Montserret, Roland; Penin, Francois; Baldus, Marc. "Solid state NMR sequential resonance assignments and conformational analysis of the 2x10.4 kDa dimeric form of the Bacillus subtilis protein Crh"  J. Biomol. NMR 27, 323-339 (2003).

Assembly members:
catabolite repression HPr, polymer, 93 residues, 10391 Da.

Natural source:   Common Name: Bacillus subtilis   Taxonomy ID: 1423   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
catabolite repression HPr: MVQQKVEVRLKTGLQARPAA LFVQEANRFTSDVFLEKDGK KVNAKSIMGLMSLAVSTGTE VTLIAQGEDEQEALEKLAAY VQEEVLQHHHHHH

Data sets:
Data typeCount
15N chemical shifts90
13C chemical shifts360

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Crh subunit 11
2Crh subunit 21

Entities:

Entity 1, Crh subunit 1 93 residues - 10391 Da.

1   METVALGLNGLNLYSVALGLUVALARGLEU
2   LYSTHRGLYLEUGLNALAARGPROALAALA
3   LEUPHEVALGLNGLUALAASNARGPHETHR
4   SERASPVALPHELEUGLULYSASPGLYLYS
5   LYSVALASNALALYSSERILEMETGLYLEU
6   METSERLEUALAVALSERTHRGLYTHRGLU
7   VALTHRLEUILEALAGLNGLYGLUASPGLU
8   GLNGLUALALEUGLULYSLEUALAALATYR
9   VALGLNGLUGLUVALLEUGLNHISHISHIS
10   HISHISHIS

Samples:

sample_1: catabolite repression HPr, [U-98% 13C; U-98% 15N], 4 – 20 mg

Ex-cond_1: pH: 6.9; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
13C-13C proton driven spin diffusion (PDSD)sample_1not availableEx-cond_1
13C-13C CO selective J-decoupled PDSDsample_1not availableEx-cond_1
13C-13C RFDRsample_1not availableEx-cond_1
13C-13C DQ SPC-5sample_1not availableEx-cond_1
15N-13C NCACOsample_1not availableEx-cond_1
15N-13C NCOCACBsample_1not availableEx-cond_1

Software:

NMRPipe v2.0 - data processing

NMRVIEW v5.0 - assignments

NMR spectrometers:

  • BRUKER DSX 500 MHz
  • BRUKER DSX 600 MHz

Related Database Links:

BMRB 4972
PDB
DBJ BAI87096 BAM55550 BAM59563 GAK79281
EMBL CAB08060 CAB15479 CCU60557 CEI59278 CEJ79135
GB ADM39440 ADV94285 AEP88381 AEP92509 AFI30049
REF NP_391354 WP_003219835 WP_024122965 WP_024713407 YP_003867749
SP O06976