BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5715

Title: Paramagnetic relaxation enhancements of the amide protons in HHP-tagged thioredoxin   PubMed: 15014231

Deposition date: 2003-03-04 Original release date: 2004-03-23

Authors: Jensen, Malene; Lauritzen, Connie; Dahl, Soren; Pedersen, John; Led, Jens

Citation: Jensen, Malene; Lauritzen, Connie; Dahl, Soren; Pedersen, John; Led, Jens. "Binding ability of a HHP-tagged protein towards Ni(2+) studied by paramagnetic NMR relaxation: The possibility of obtaining long-range structure information."  J. Biomol. NMR 29, 175-185 (2004).

Assembly members:
HHP-tagged E. coli Thioredoxin, polymer, 111 residues, Formula weight is not available
NI, non-polymer, 58.693 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
HHP-tagged E. coli Thioredoxin: HHPSDKIIHLTDDSFDTDVL KADGAILVDFWAEWCGPCKM IAPILDEIADEYQGKLTVAK LNIDQNPGTAPKYGIRGIPT LLLFKNGEVAATKVGALSKG QLKEFLDANLA

Data sets:
  • assigned_chemical_shifts
  • heteronucl_T1_relaxation
Data typeCount
T1 relaxation values84

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HHP-Trx unit 11
2HHP-Trx unit 21
3HHP-Trx unit 31
4Nickel ion2

Entities:

Entity 1, HHP-Trx unit 1 111 residues - Formula weight is not available

1   HISHISPROSERASPLYSILEILEHISLEU
2   THRASPASPSERPHEASPTHRASPVALLEU
3   LYSALAASPGLYALAILELEUVALASPPHE
4   TRPALAGLUTRPCYSGLYPROCYSLYSMET
5   ILEALAPROILELEUASPGLUILEALAASP
6   GLUTYRGLNGLYLYSLEUTHRVALALALYS
7   LEUASNILEASPGLNASNPROGLYTHRALA
8   PROLYSTYRGLYILEARGGLYILEPROTHR
9   LEULEULEUPHELYSASNGLYGLUVALALA
10   ALATHRLYSVALGLYALALEUSERLYSGLY
11   GLNLEULYSGLUPHELEUASPALAASNLEU
12   ALA

Entity 2, Nickel ion - Ni - 58.693 Da.

1   NI

Samples:

sample_1: HHP-tagged E. coli Thioredoxin, [U-15N], 1.0 mM

sample_2: HHP-tagged E. coli Thioredoxin, [U-15N], 1.0 mM; Nickel ion 0.1 mM

sample_3: HHP-tagged E. coli Thioredoxin, [U-15N], 1.0 mM; Nickel ion 0.2 mM

sample_4: HHP-tagged E. coli Thioredoxin, [U-15N], 1.0 mM; Nickel ion 0.3 mM

sample_5: HHP-tagged E. coli Thioredoxin, [U-15N], 1.0 mM; Nickel ion 0.4 mM

sample_6: HHP-tagged E. coli Thioredoxin, [U-15N], 1.0 mM; Nickel ion 0.5 mM

sample_7: HHP-tagged E. coli Thioredoxin, [U-15N], 1.0 mM; Nickel ion 0.6 mM

sample_8: HHP-tagged E. coli Thioredoxin, [U-15N], 1.0 mM; Nickel ion 0.7 mM

sample_9: HHP-tagged E. coli Thioredoxin, [U-15N], 1.0 mM; Nickel ion 0.8 mM

sample_10: HHP-tagged E. coli Thioredoxin, [U-15N], 1.0 mM; Nickel ion 0.9 mM

sample_11: HHP-tagged E. coli Thioredoxin, [U-15N], 1.0 mM; Nickel ion 1.0 mM

Condition_1: pH: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
Inversion recovery 1H-15N HSQC experimentsnot availablenot availableCondition_1

Software:

No software information available

NMR spectrometers:

  • Varian Inova 500 MHz

Related Database Links:

BMRB 17700 1812 1813 62
PDB
DBJ BAA00903 BAB38137 BAE77517 BAG79587 BAH61053
EMBL CAA79851 CAD09400 CAP78228 CAQ34125 CAQ91183
GB AAA24533 AAA24534 AAA24693 AAA24694 AAA24696
PIR AF0922 B91218 C86064
REF NP_290411 NP_312741 NP_418228 NP_457831 NP_462806
SP P0AA25 P0AA26 P0AA27 P0AA28 P0AA29