BMRB Entry 5693

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5693

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Title:
Backbone chemical shift assignments of Grb2 complexed with ligand peptides for SH3 and SH2 domains
Deposition date:
2003-02-13
Original release date:
2003-08-07
Authors:
Yuzawa, Satoru; Ogura, Kenji; Hatanaka, Hideki; Miura, Kin-ichiro; Inagaki, Fuyuhiko
Citation:

Citation: Yuzawa, Satoru; Ogura, Kenji; Hatanaka, Hideki; Miura, Kin-ichiro; Inagaki, Fuyuhiko. "Letter to the Editor: Backbone assignments of Grb2 complexed with ligand peptides for SH3 and SH2 domains"  J. Biomol. NMR 27, 185-186 (2003).

Assembly members:

Assembly members:
growth factor receptor-bound protein 2, polymer, 217 residues, 25157 Da.
EGF Receptor phosphorylated peptide, polymer, 7 residues, Formula weight is not available
Sos peptide, polymer, 10 residues, 1170 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET3a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
growth factor receptor-bound protein 2: MEAIAKYDFKATADDELSFK RGDILKVLNEESDQNWYKAE LNGKDGFIPKNYIEMKPHPW FFGKIPRAKAEEMLSKQRHD GAFLIRESESAPGDFSLSVK FGNDVQHFKVLRDGAGKYFL WVVKFNSLNELVDYHRSTSV SRNQQIFLRDIEQVPQQPTY VQALFDFDPQEDGELGFRRG DFIHVMDNSDPNWWKGAAHG QTGMFPRNYVTPVNRNV
EGF Receptor phosphorylated peptide: EXINSQV
Sos peptide: VPPPVPPRRR

Data sets:
Data typeCount
1H chemical shifts190
13C chemical shifts608
15N chemical shifts190

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Grb21
2EGF Receptor phosphorylated peptide2
3Sos peptide3

Entities:

Entity 1, Grb2 217 residues - 25157 Da.

1   METGLUALAILEALALYSTYRASPPHELYS
2   ALATHRALAASPASPGLULEUSERPHELYS
3   ARGGLYASPILELEULYSVALLEUASNGLU
4   GLUSERASPGLNASNTRPTYRLYSALAGLU
5   LEUASNGLYLYSASPGLYPHEILEPROLYS
6   ASNTYRILEGLUMETLYSPROHISPROTRP
7   PHEPHEGLYLYSILEPROARGALALYSALA
8   GLUGLUMETLEUSERLYSGLNARGHISASP
9   GLYALAPHELEUILEARGGLUSERGLUSER
10   ALAPROGLYASPPHESERLEUSERVALLYS
11   PHEGLYASNASPVALGLNHISPHELYSVAL
12   LEUARGASPGLYALAGLYLYSTYRPHELEU
13   TRPVALVALLYSPHEASNSERLEUASNGLU
14   LEUVALASPTYRHISARGSERTHRSERVAL
15   SERARGASNGLNGLNILEPHELEUARGASP
16   ILEGLUGLNVALPROGLNGLNPROTHRTYR
17   VALGLNALALEUPHEASPPHEASPPROGLN
18   GLUASPGLYGLULEUGLYPHEARGARGGLY
19   ASPPHEILEHISVALMETASPASNSERASP
20   PROASNTRPTRPLYSGLYALAALAHISGLY
21   GLNTHRGLYMETPHEPROARGASNTYRVAL
22   THRPROVALASNARGASNVAL

Entity 2, EGF Receptor phosphorylated peptide 7 residues - Formula weight is not available

1   GLUPTRILEASNSERGLNVAL

Entity 3, Sos peptide 10 residues - 1170 Da.

1   VALPROPROPROVALPROPROARGARGARG

Samples:

sample_1: growth factor receptor-bound protein 2, [U-80% 2H; U-98% 13C; U-98% 15N], 1.2 mM; EGF Receptor phosphorylated peptide 1.44 mM; Sos peptide 6.0 mM

Ex-cond_1: pH: 7.2; temperature: 298 K; ionic strength: 0.03 M

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCsample_1not availableEx-cond_1
HNCOsample_1not availableEx-cond_1
HN(CA)COsample_1not availableEx-cond_1
HNCAsample_1not availableEx-cond_1
HN(CO)CAsample_1not availableEx-cond_1
NH(COCA)CBsample_1not availableEx-cond_1
HN(CA)CBsample_1not availableEx-cond_1

Software:

nmrPipe - raw spectral data processing

NMR spectrometers:

  • Varian INOVAplus 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks