BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5692

Title: Structural and functional insights into PINCH LIM4 domain-mediated integrin signaling   PubMed: 12794636

Deposition date: 2003-02-12 Original release date: 2003-08-08

Authors: Velyvis, Algirdas; Vaynberg, Julie; Vinogradova, Olga; Zhang, Yongjun; Wu, Chuanyue; Qin, Jun

Citation: Velyvis, Algirdas; Vaynberg, Julie; Yang, Yanwu; Vinogradova, Olga; Zhang, Yongjun; Wu, Chuanyue; Qin, Jun. "Structural and Functional Insights into PINCH LIM4 Domain-mediated Integrin Signaling"  Nat. Struct. Biol. 10, 558-564 (2003).

Assembly members:
4th LIM domain of PINCH protein, polymer, 66 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
4th LIM domain of PINCH protein: GSMGVPICGACRRPIEGRVV NAMGKQWHVEHFVCAKCEKP FLGHRHYERKGLAYCETHYN QLFGDV

Data sets:
Data typeCount
13C chemical shifts227
15N chemical shifts71
1H chemical shifts446

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1LIM41
2Zinc ion (II)2

Entities:

Entity 1, LIM4 66 residues - Formula weight is not available

1   GLYSERMETGLYVALPROILECYSGLYALA
2   CYSARGARGPROILEGLUGLYARGVALVAL
3   ASNALAMETGLYLYSGLNTRPHISVALGLU
4   HISPHEVALCYSALALYSCYSGLULYSPRO
5   PHELEUGLYHISARGHISTYRGLUARGLYS
6   GLYLEUALATYRCYSGLUTHRHISTYRASN
7   GLNLEUPHEGLYASPVAL

Entity 2, Zinc ion (II) - Zn - 65.409 Da.

1   ZN

Samples:

the_sample: 4th LIM domain of PINCH protein, [U-95% 13C; U-95% 15N], 1.7 mM; NaCl 50 mM; Na2HPO4 25 mM

the_conditions: ionic strength: 125 mM; pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCthe_samplenot availablethe_conditions
HNCACBthe_samplenot availablethe_conditions
HNCOthe_samplenot availablethe_conditions
N,C-edited-NOESYthe_samplenot availablethe_conditions
HNHAthe_samplenot availablethe_conditions
HCCH-TOCSYthe_samplenot availablethe_conditions

Software:

NMRPipe -

NMR spectrometers:

  • Varian INOVA 500 MHz

Related Database Links:

PDB
GB AGW21640 EFB20618 ELW62207 KFP50847 KFP56517
REF XP_002832613 XP_002930391 XP_004005972 XP_004938580 XP_005953270

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts