BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5687

Title: Stably folded de novo proteins from a designed combinatorial library

Authors: Hecht, Michael; Wei, Yinan

Citation: Wei, Yinan; Fela, David; Kim, Seho; Hecht, Michael; Baum, Jean. "Letter to the Editor: 1H, 13C and 15N resonance assignments of S-824, a de novo four_helix bundle from a designed combinatorial library"  J. Biomol. NMR 27, 395-396 (2003).

Assembly members:
de novo protein, polymer, 102 residues, 11928 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
de novo protein: MYGKLNDLLEDLQEVLKNLH KNWHGGKDNLHDVDNHLQNV IEDIHDFMQGGGSGGKLQEM MKEFQQVLDELNNHLQGGKH TVHHIEQNIKEIFHHLEELV HR

Data sets:
Data typeCount
13C chemical shifts414
15N chemical shifts119
1H chemical shifts718
T1 relaxation values202
T2 relaxation values202

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1s824 monomer1

Entities:

Entity 1, s824 monomer 102 residues - 11928 Da.

1   METTYRGLYLYSLEUASNASPLEULEUGLU
2   ASPLEUGLNGLUVALLEULYSASNLEUHIS
3   LYSASNTRPHISGLYGLYLYSASPASNLEU
4   HISASPVALASPASNHISLEUGLNASNVAL
5   ILEGLUASPILEHISASPPHEMETGLNGLY
6   GLYGLYSERGLYGLYLYSLEUGLNGLUMET
7   METLYSGLUPHEGLNGLNVALLEUASPGLU
8   LEUASNASNHISLEUGLNGLYGLYLYSHIS
9   THRVALHISHISILEGLUGLNASNILELYS
10   GLUILEPHEHISHISLEUGLUGLULEUVAL
11   HISARG

Samples:

sample_1: de novo protein, [U-13C; U-15N], 2.0 mM

Ex-cond_1: ionic strength: 0.05 M; pH: 4.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYsample_1not availableEx-cond_1
1H-13C NOESYsample_1not availableEx-cond_1
HNCAsample_1not availableEx-cond_1
HN(CO)CAsample_1not availableEx-cond_1
HNCOsample_1not availableEx-cond_1
HN(CA)COsample_1not availableEx-cond_1
CBCANHsample_1not availableEx-cond_1
CBCA(CO)NHsample_1not availableEx-cond_1
1H-15N TOCSYsample_1not availableEx-cond_1
1H-13C TOCSYsample_1not availableEx-cond_1

Software:

No software information available

NMR spectrometers:

  • Varian INOVA 600 MHz

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