BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5685

Title: 1H, 15N, and 13C Assignments and CSI Analysis of a 18kDa Complex Formed by the Carboxyl-Terminal Domains of RAP74 and FCP1   PubMed: 12578358

Authors: Nguyen, Bao; Omichinski, James; Legault, Pascale

Citation: Nguyen, Bao; Chen, H.; Kobor, M.; Greenblatt, J.; Legault, Pascale; Omichinski, James. "Solution structure of the carboxyl-terminal domain of RAP74 and NMR characterization of the FCP1-binding sites of RAP74 and human TFIIB"  Biochemistry 42, 1460-1469 (2003).

Assembly members:
RAP74, polymer, 82 residues, Formula weight is not available
FCP1, polymer, 83 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
RAP74: STPQPPSGKTTPNSGDVQVT EDAVRRYLTRKPMTTKDLLK KFQTKKTGLSSEQTVNVLAQ ILKRLNPERKMINDKMHFSL KE
FCP1: PEEQEEEPQPRKPGTRRERT LGAPASSERSAAGGRGPRGH KRKLNEEDAASESSRESSNE DEGSSSEADEMAKALEAELN DLM

Data sets:
Data typeCount
1H chemical shifts833
13C chemical shifts572
15N chemical shifts139

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RAP741
2FCP12

Entities:

Entity 1, RAP74 82 residues - Formula weight is not available

1   SERTHRPROGLNPROPROSERGLYLYSTHR
2   THRPROASNSERGLYASPVALGLNVALTHR
3   GLUASPALAVALARGARGTYRLEUTHRARG
4   LYSPROMETTHRTHRLYSASPLEULEULYS
5   LYSPHEGLNTHRLYSLYSTHRGLYLEUSER
6   SERGLUGLNTHRVALASNVALLEUALAGLN
7   ILELEULYSARGLEUASNPROGLUARGLYS
8   METILEASNASPLYSMETHISPHESERLEU
9   LYSGLU

Entity 2, FCP1 83 residues - Formula weight is not available

1   PROGLUGLUGLNGLUGLUGLUPROGLNPRO
2   ARGLYSPROGLYTHRARGARGGLUARGTHR
3   LEUGLYALAPROALASERSERGLUARGSER
4   ALAALAGLYGLYARGGLYPROARGGLYHIS
5   LYSARGLYSLEUASNGLUGLUASPALAALA
6   SERGLUSERSERARGGLUSERSERASNGLU
7   ASPGLUGLYSERSERSERGLUALAASPGLU
8   METALALYSALALEUGLUALAGLULEUASN
9   ASPLEUMET

Samples:

sample_1: RAP74, [U-95% 13C; U-95% 15N], 1.0 mM; FCP1, [U-95% 13C; U-95% 15N], 1.0 mM

Ex-cond_1: pH: 6.5; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
1H-13C CT-HSQCnot availablenot availablenot available
1H-15N NOESY-HSQCnot availablenot availablenot available
HNCACBnot availablenot availablenot available
(HB)CBCA(CO)NNHnot availablenot availablenot available
HNCOnot availablenot availablenot available
H(CCO)NNH-TOCSYnot availablenot availablenot available
C(CO)NNH-TOCSYnot availablenot availablenot available
1H-13C HMQC-NOESYnot availablenot availablenot available
NOESYnot availablenot availablenot available
DQF-COSYnot availablenot availablenot available

Software:

nmrview v5.1 -

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz

Related Database Links:

BMRB 16296
PDB
GB AAC64549 AAH52576 EAW66628
REF NP_001189433 NP_004706 XP_002828379 XP_003805250 XP_004059621 XP_001144243 XP_001144161 NP_004706
SP Q9Y5B0
GenBank EAW66628 AAH52576 AAC64549