BMRB Entry 5655

Name: U80G U6 ISL RNA Chemical Shifts
Published: 2009-06-02

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PDB ID: 1nc0
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5655
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

U80G U6 ISL RNA Chemical Shifts

Deposition date:

2002-12-05

Original release date:

2009-06-02

Authors:

Sashital, Dipali; Allmann, Anne; Van Doren, Steve; Butcher, Samuel

Citation:

Citation: Sashital, D.; Allmann, A.; Van Doren, S.; Butcher, S.. "Structural basis for a lethal mutation in U6 RNA" Biochemistry 42, 1470-1477 (2003).
PubMed: 12578359

Assembly members:

Assembly members:
U6 ISL RNA, polymer, 24 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
U6 ISL RNA: GGUUCCCCUGCAUAAGGAGG AACC

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
13C chemical shifts	138
15N chemical shifts	15
1H chemical shifts	203

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	U80G U6 ISL RNA	1

Entities:

Entity 1, U80G U6 ISL RNA 24 residues - Formula weight is not available

1

G

U

C

U

G

2

C

A

U

A

G

A

G

3

A

C

Samples:

sample_1: U6 ISL RNA, [U-13C; U-15N], 1.5 mM

cond_1: pH: 6.5; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
not available	sample_1	not available	cond_1

Software:

CNS v1.1 -

NMR spectrometers:

Bruker DMX 400 MHz
Bruker DMX 500 MHz
Bruker DMX 600 MHz
Bruker DMX 750 MHz