BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5630

Title: 1H 13C and 15N Assigned Chemical Shifts for ER75, an NESG target   PubMed: 15930006

Authors: Liu, Gaohua; Szyperski, Thomas; Montelione, Gaetano; Chiang, Yiwen; Acton, Thomas

Citation: Liu, Gaohua; Chiang, Yiwen; Acton, Thomas; Li, Z.; Murray, D.; Montelione, Gaetano; Szyperski, Thomas. "High-quality homology models derived from NMR and X-ray structures of E. coli proteins YgdK and Suf E suggest that all members of the YgdK/Suf E protein family are enhancers of cysteine desulfurases"  Protein Sci. 14, 1597-1608 (2005).

Assembly members:
er75, polymer, 149 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
er75: MTNPQFAGHPFGTTVTAETL RNTFAPLTQWEDKYRQLIML GKQLPALPDELKAQAKEIAG CENRVWLGYTVAENGKMHFF GDSEGRIVRGLLAVLLTAVE GKTAAELQAQSPLALFDELG LRAQLSASRSQGLNALSEAI IAATKQVLE

Data sets:
Data typeCount
1H chemical shifts1058
13C chemical shifts612
15N chemical shifts161

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1er75 monomer1

Entities:

Entity 1, er75 monomer 149 residues - Formula weight is not available

1   METTHRASNPROGLNPHEALAGLYHISPRO
2   PHEGLYTHRTHRVALTHRALAGLUTHRLEU
3   ARGASNTHRPHEALAPROLEUTHRGLNTRP
4   GLUASPLYSTYRARGGLNLEUILEMETLEU
5   GLYLYSGLNLEUPROALALEUPROASPGLU
6   LEULYSALAGLNALALYSGLUILEALAGLY
7   CYSGLUASNARGVALTRPLEUGLYTYRTHR
8   VALALAGLUASNGLYLYSMETHISPHEPHE
9   GLYASPSERGLUGLYARGILEVALARGGLY
10   LEULEUALAVALLEULEUTHRALAVALGLU
11   GLYLYSTHRALAALAGLULEUGLNALAGLN
12   SERPROLEUALALEUPHEASPGLULEUGLY
13   LEUARGALAGLNLEUSERALASERARGSER
14   GLNGLYLEUASNALALEUSERGLUALAILE
15   ILEALAALATHRLYSGLNVALLEUGLU

Samples:

sample_1: er75, [U-15N], 1.0 mM

sample_2: er75, [U-13C; U-15N], 1.0 mM

Ex-cond_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Varian Inova 750 MHz
  • Varian Inova 600 MHz

Related Database Links:

PDB
DBJ BAB37094 BAE76883 BAG78595 BAI27072 BAI32101
EMBL CAP77244 CAQ33140 CAQ99739 CAR04321 CAR09425
GB AAB40461 AAC75853 AAG57925 AAN44312 AAN81827
REF NP_289366 NP_311698 NP_417291 NP_708605 NP_755257
SP P0AGF2 P0AGF3