BMRB Entry 5605

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5605

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Title:
Sequence-specific 1H, 13C and 15N resonance assignments of SAM22, an allergenic stress-induced protein from soy bean
Deposition date:
2002-11-30
Original release date:
2004-05-24
Authors:
Neudecker, Philipp; Lehmann, Katrin; Roesch, Paul
Citation:

Citation: Neudecker, Philipp; Lehmann, Katrin; Roesch, Paul. "Letter to the Editor: Sequence-specific 1H, 13C and 15N resonance assignments of SAM22, an allergenic stress-induced protein from soy bean"  J. Biomol. NMR 26, 191-192 (2003).

Assembly members:

Assembly members:
SAM22, polymer, 157 residues, 16641 Da.

Natural source:

Natural source:   Common Name: soybean   Taxonomy ID: 3847   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Glycine max

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SAM22: GVFTFEDEINSPVAPATLYK ALVTDADNVIPKALDSFKSV ENVEGNGGPGTIKKITFLED GETKFVLHKIESIDEANLGY SYSVVGGAALPDTAEKITFD SKLVAGPNGGSAGKLTVKYE TKGDAEPNQDELKTGKAKAD ALFKAIEAYLLAHPDYN

Data sets:
Data typeCount
13C chemical shifts630
15N chemical shifts157
1H chemical shifts1076
coupling constants89

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SAM221

Entities:

Entity 1, SAM22 157 residues - 16641 Da.

1   GLYVALPHETHRPHEGLUASPGLUILEASN
2   SERPROVALALAPROALATHRLEUTYRLYS
3   ALALEUVALTHRASPALAASPASNVALILE
4   PROLYSALALEUASPSERPHELYSSERVAL
5   GLUASNVALGLUGLYASNGLYGLYPROGLY
6   THRILELYSLYSILETHRPHELEUGLUASP
7   GLYGLUTHRLYSPHEVALLEUHISLYSILE
8   GLUSERILEASPGLUALAASNLEUGLYTYR
9   SERTYRSERVALVALGLYGLYALAALALEU
10   PROASPTHRALAGLULYSILETHRPHEASP
11   SERLYSLEUVALALAGLYPROASNGLYGLY
12   SERALAGLYLYSLEUTHRVALLYSTYRGLU
13   THRLYSGLYASPALAGLUPROASNGLNASP
14   GLULEULYSTHRGLYLYSALALYSALAASP
15   ALALEUPHELYSALAILEGLUALATYRLEU
16   LEUALAHISPROASPTYRASN

Samples:

sample_1: SAM22, [U-99% 15N], 1.5 mM

sample_2: SAM22, [U-99% 13C; U-99% 15N], 0.7 – 1.5 mM

cond_1: pH: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D TOCSYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
1H,15N HSQCnot availablenot availablenot available
HNHAnot availablenot availablenot available
3D 1H,15N-TOCSY-HSQCnot availablenot availablenot available
3D 1H,15N-NOESY-HSQCnot availablenot availablenot available
3D 1H,15N/1H,15N-HMQC-NOESY-HSQCnot availablenot availablenot available
1H,13C CTHSQCnot availablenot availablenot available
HNCOnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HBHA(CBCACO)NHnot availablenot availablenot available
C(CO)NHnot availablenot availablenot available
H(CCO)NHnot availablenot availablenot available
H(C)CH-COSYnot availablenot availablenot available
(H)CCH-COSYnot availablenot availablenot available
HC(C)H-TOCSYnot availablenot availablenot available
3D 1H,13C-NOESY-HSQCnot availablenot availablenot available
3D 1H,13C/1H,15N-HMQC-NOESY-HSQCnot availablenot availablenot available

Software:

NDEE - spectral analysis

NMRView v5.0.4 - spectral analysis

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DMX 750 MHz

Related Database Links:

PDB
EMBL CAA42646 CAC18803
GB ABB73065 ACU15400 ADR51747 KHN02927 KHN02928
REF NP_001236038 XP_006582821
SP P26987
AlphaFold P26987

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks