BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5569

Title: 15N relaxation rates for backbone of GB1   PubMed: 12473458

Authors: Idiyatullin, Djaudat; Nesmelova, Irina; Daragan, Vladimir; Mayo, Kevin

Citation: Idiyatullin, Djaudat; Nesmelova, Irina; Daragan, Vladimir; Mayo, Kevin. "Heat Capacities and a Snapshot of the Energy Landscape in Protein GB1 from the Pre-denaturation Temperature Dependence of Backbone NH Nanosecond Fluctuations"  J. Mol. Biol. 325, 149-162 (2003).

Assembly members:
immunoglobulin-binding domain, polymer, 56 residues, Formula weight is not available

Natural source:   Common Name: Streptococcus   Taxonomy ID: 1301   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Streptococcus not available

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
immunoglobulin-binding domain: MTYKLILNGKTLKGETTTEA VDAATAEKVFKQYANDNGVD GEWTYDDATKTFTVTE

Data sets:
Data typeCount
T1 relaxation values1680
T2 relaxation values1680

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GB1 monomer1

Entities:

Entity 1, GB1 monomer 56 residues - Formula weight is not available

1   METTHRTYRLYSLEUILELEUASNGLYLYS
2   THRLEULYSGLYGLUTHRTHRTHRGLUALA
3   VALASPALAALATHRALAGLULYSVALPHE
4   LYSGLNTYRALAASNASPASNGLYVALASP
5   GLYGLUTRPTHRTYRASPASPALATHRLYS
6   THRPHETHRVALTHRGLU

Samples:

sample_1: immunoglobulin-binding domain, [U-15N], 10 – 10 mg/mL

Ex-cond_1: pH: 5.25; temperature: 278 K

Ex-cond_2: pH: 5.25; temperature: 283 K

Ex-cond_3: pH: 5.25; temperature: 288 K

Ex-cond_4: pH: 5.25; temperature: 293 K

Ex-cond_5: pH: 5.25; temperature: 298 K

Ex-cond_6: pH: 5.25; temperature: 303 K

Ex-cond_7: pH: 5.25; temperature: 308 K

Ex-cond_8: pH: 5.25; temperature: 313 K

Ex-cond_9: pH: 5.25; temperature: 318 K

Ex-cond_10: pH: 5.25; temperature: 323 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
Steady-state {1H}-15N NOEnot availablenot availablenot available

Software:

NMRView - calculating relaxation rates, peak assignments

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz

Related Database Links:

BMRB 15156 15380 16444 16627 16670 16755 16873 16882 16958 17810 18397 19394 6280 6988 7280
PDB
EMBL CAA37410