BMRB Entry 5561

Title:
NMR solution structure of the activation domain of human procarboxipeptidase A2
Deposition date:
2002-10-17
Original release date:
2003-02-21
Authors:
Jimenez, M. Angeles; Villegas, Virtudes; Santoro, Jorge; Serrano, Luis; Vendrell, Josep; Aviles, Xavier; Rico, Manuel
Citation:

Citation: Jimenez, M. Angeles; Villegas, Virtudes; Santoro, Jorge; Serrano, Luis; Vendrell, Josep; Aviles, F. Xavier; Rico, Manuel. "NMR Solution Structure of the Activation Domain of Human Procarboxipeptidase A2"  Protein Sci. 12, 296-305 (2003).
PubMed: 12538893

Assembly members:

Assembly members:
Activation domain of human procarboxipeptidase, polymer, 81 residues, 9212 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTZ18U

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Activation domain of human procarboxipeptidase: MRSLETFVGDQVLEIVPSNE EQIKNLLQLEAQEHLQLDFW KSPTTPGETAHVRVPFVNVQ AVKVFLESQGIAYSIMIEDV Q

Data sets:
Data typeCount
15N chemical shifts87
1H chemical shifts599

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ADA2h1

Entities:

Entity 1, ADA2h 81 residues - 9212 Da.

1   METARGSERLEUGLUTHRPHEVALGLYASP
2   GLNVALLEUGLUILEVALPROSERASNGLU
3   GLUGLNILELYSASNLEULEUGLNLEUGLU
4   ALAGLNGLUHISLEUGLNLEUASPPHETRP
5   LYSSERPROTHRTHRPROGLYGLUTHRALA
6   HISVALARGVALPROPHEVALASNVALGLN
7   ALAVALLYSVALPHELEUGLUSERGLNGLY
8   ILEALATYRSERILEMETILEGLUASPVAL
9   GLN

Samples:

sample-1: Activation domain of human procarboxipeptidase 2.0 mM; sodium phosphate buffer 50 mM

sample-2: Activation domain of human procarboxipeptidase, [U-95.5% 15N], 2.0 mM; sodium phosphate buffer 50 mM

Exp_cond-1: pH: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H COSYnot availablenot availablenot available
2D 1H-1H TOCSYnot availablenot availablenot available
2D 1H-1H NOESYnot availablenot availablenot available
2D 1H-15N HSQCnot availablenot availablenot available
3D 1H-1H-15N NOESYnot availablenot availablenot available
3D 1H-1H-15N TOCSYnot availablenot availablenot available
2D 1H-13C HSQCnot availablenot availablenot available

Software:

XEASY -

NMR spectrometers:

  • Bruker AMX 600 MHz

Related Database Links:

PDB
DBJ BAG56890
GB AAA74425 AAH07009 AAH14571 AAH15140 AAP36067
REF NP_001860 XP_001156440 XP_002818494 XP_003813529
SP P48052
AlphaFold P48052

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks