BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5549

Title: Neuropeptide Y5-Receptor II: Solution Structure and Dynamics of [31Ala,32Pro]-NPY   PubMed: 12069594

Authors: Bader, Reto; Rytz, G.; Lerch, M.; Beck-Sickinger, Annette; Zerbe, Oliver

Citation: Bader, Reto; Rytz, G.; Lerch, M.; Beck-Sickinger, Annette; Zerbe, Oliver. "Key Motif to Gain Selectivity at the Neuropeptide Y5-receptor: Structure and Dynamics of Micelle-bound [Ala31,Pro32]-NPY"  Biochemistry 41, 8031-8042 (2002).

Assembly members:
[Ala31, Pro32]-NEUROPEPTIDE Y, polymer, 37 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
[Ala31, Pro32]-NEUROPEPTIDE Y: YPSKPDNPGEDAPAEDLARY YSALRHYINLAPRQRYX

Data sets:
Data typeCount
1H chemical shifts222
15N chemical shifts31
T1 relaxation values56
T2 relaxation values54
heteronuclear NOE values28
S2 parameters28

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1[Ala31, Pro32]-NPY1

Entities:

Entity 1, [Ala31, Pro32]-NPY 37 residues - Formula weight is not available

1   TYRPROSERLYSPROASPASNPROGLYGLU
2   ASPALAPROALAGLUASPLEUALAARGTYR
3   TYRSERALALEUARGHISTYRILEASNLEU
4   ALAPROARGGLNARGTYRNH2

Samples:

sample_1: [Ala31, Pro32]-NEUROPEPTIDE Y, [U-15N], 2.5 mM; DPC 300 mM; H2O 90%; D2O 90%

sample_cond_1: pH*: 6.0; temperature: 310 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1not availablesample_cond_1
E-COSYsample_1not availablesample_cond_1

Software:

XWINNMR v2.1 - data processing

XEASY v1.53 - data analysis

DYANA v1.5 - structure solution

NMR spectrometers:

  • Varian UnityPlus 500 MHz
  • Bruker AVANCE 600 MHz

Related Database Links:

BMRB 4862
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