BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5505

Title: Backbone Dynamics of Reduced Plastocyanin from the Cyanobacterium Anabaena variabilis: Regions Involved in Electron Transfer have Enhanched Mobility   PubMed: 12525159

Authors: Ma, Lixin; Hass, Mathias; Rasmussen, Nanna; Kristensen, Soren; Ulstrup, Jens; Led, Jens

Citation: Ma, Lixin; Hass, Mathias; Vierick, Nanna; Kristensen, Soren; Ulstrup, Jens; Led, Jens. "Backbone Dynamics of Reduced Plastocyanin from the Cyanobacterium Anabaena variabilis: Regions Involved in Electron Transfer have Enhanched Mobility"  Biochemistry 42, 320-330 (2003).

Assembly members:
Cu(II) Plastocyanin from Anabeana Variabilis, polymer, 105 residues, 10500 Da.
COPPER (I) ION, non-polymer, 63.546 Da.

Natural source:   Common Name: Anabaena variabilis   Taxonomy ID: 1172   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Anabaena variabilis

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Cu(II) Plastocyanin from Anabeana Variabilis: ETYTVKLGSDKGLLVFEPAK LTIKPGDTVEFLNNKVPPHN VVFDAALNPAKSADLAKSLS HKQLLMSPGQSTSTTFPADA PAGEYTFYCEPHRGAGMVGK ITVAG

Data sets:
Data typeCount
1H chemical shifts673
13C chemical shifts429
15N chemical shifts104
T1 relaxation values163
T2 relaxation values163

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Plastocyanin1
2COPPER (I) ION2

Entities:

Entity 1, Plastocyanin 105 residues - 10500 Da.

1   GLUTHRTYRTHRVALLYSLEUGLYSERASP
2   LYSGLYLEULEUVALPHEGLUPROALALYS
3   LEUTHRILELYSPROGLYASPTHRVALGLU
4   PHELEUASNASNLYSVALPROPROHISASN
5   VALVALPHEASPALAALALEUASNPROALA
6   LYSSERALAASPLEUALALYSSERLEUSER
7   HISLYSGLNLEULEUMETSERPROGLYGLN
8   SERTHRSERTHRTHRPHEPROALAASPALA
9   PROALAGLYGLUTYRTHRPHETYRCYSGLU
10   PROHISARGGLYALAGLYMETVALGLYLYS
11   ILETHRVALALAGLY

Entity 2, COPPER (I) ION - Cu - 63.546 Da.

1   CU1

Samples:

sample_1: Cu(II) Plastocyanin from Anabeana Variabilis, [U-95% 15N], 4.1 mM; COPPER (I) ION 4.1 mM; NaCl 100 mM; H2O 90%; D2O 10%; sodium ascorbate0.6 – 0.7 mM

sample_2: Cu(II) Plastocyanin from Anabeana Variabilis, [U-95% 15N], 2.8 – 3.2 mM; COPPER (I) ION2.8 – 3.2 mM; H2O 90%; D2O 10%

cond_set_1: pH: 7.0 na; temperature: 298 K; ionic strength: 100 mM

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCnot availablenot availablenot available
HSQC-TOCSYnot availablenot availablenot available
HNCAnot availablenot availablenot available
1H-13C HSQCnot availablenot availablenot available
HSQCnot availablenot availablenot available
HMQC-COSYnot availablenot availablenot available
HNCOnot availablenot availablenot available
3D TOCSY-HMQCnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Varian UnityInova 500 MHz
  • Varian UnityInova 750 MHz
  • Bruker AMX 500 MHz

Related Database Links:

BMRB 4738 5858
PDB
DBJ BAB77782
EMBL CAA05338 CAA32527
GB AAA59364 ABA22358
PIR CUAI S06999
REF NP_484302 WP_010994435 WP_011319499 YP_323253
SP O52830 P0C178 P46444 Q3M9H8