BMRB Entry 5484

Title:
Backbone NMR assignments of the metal-free UreE from Bacillus pasteurii
Deposition date:
2002-07-31
Original release date:
2002-12-23
Authors:
Lee, Yeon-Hee; Won, Hyung-Sik; Ahn, Hee-Chul; Park, Sangho; Lee, Bong-Jin
Citation:

Citation: Lee, Yeon-Hee; Won, Hyung-Sik; Ahn, Hee-Chul; Park, Sangho; Yagi, H.; Akutsu, H.; Lee, Bong-Jin. "Letter to the Editor: Backbone NMR assignments of the metal-free UreE from Bacillus pasteurii"  J. Biomol. NMR 24, 361-362 (2002).

Assembly members:

Assembly members:
Bacillus pasteurii UreE, polymer, 147 residues, 34768 Da.

Natural source:

Natural source:   Common Name: Bacillus pasteurii   Taxonomy ID: 1474   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Bacillus pasteurii

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts433
15N chemical shifts142
1H chemical shifts142

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1BpUreE subunit 11
2BpUreE subunit 21

Entities:

Entity 1, BpUreE subunit 1 147 residues - 34768 Da.

1   METLEUILETHRLYSILEVALGLYHISILE
2   ASPASPTYRGLUSERSERASPLYSLYSVAL
3   ASPTRPLEUGLUVALGLUTRPGLUASPLEU
4   ASNLYSARGILELEUARGLYSGLUTHRGLU
5   ASNGLYTHRASPILEALAILELYSLEUGLU
6   ASNSERGLYTHRLEUARGTYRGLYASPVAL
7   LEUTYRGLUSERASPASPTHRLEUILEALA
8   ILEARGTHRLYSLEUGLULYSVALTYRVAL
9   ILELYSPROGLNTHRMETGLNGLUMETGLY
10   LYSMETALAPHEGLUILEGLYASNARGHIS
11   THRMETCYSILEILEGLUASPASPGLUILE
12   LEUVALARGTYRASPLYSTHRLEUGLULYS
13   LEUILEASPGLUVALGLYVALSERTYRGLU
14   GLNSERGLUARGARGPHELYSGLUPROPHE
15   LYSTYRARGGLYHISGLNHIS

Samples:

sample_1: Bacillus pasteurii UreE, [U-90% 2H; U-13C; U-15N], 1.4 mM

Ex-cond_1: pH: 6.5; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
[1H/15N]HSQCnot availablenot availablenot available
15N-edited NOESY-HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HN(CA)CBnot availablenot availablenot available
HN(COCA)CBnot availablenot availablenot available
HNCOnot availablenot availablenot available
HNCACOnot availablenot availablenot available

Software:

NMRPipe -

NMRView -

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 800 MHz

Related Database Links:

BMRB 5826
PDB
GB AAA73987 AAD55059
SP P50049
AlphaFold P50049

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks